Mechanical stress in periodontium ; Cellular and molecular biological study and Clinical implication

牙周组织的机械应力；

基本信息

批准号：
07557283
负责人：
MIKI Mirei
金额：
$ 1.66万
依托单位：
TOHOKU UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (A)
财政年份：
1995
资助国家：
日本
起止时间：
1995 至 1997
项目状态：
已结题

项目摘要

1.Osteoblast-like cells, MC3T3-El or periodontal-ligament derived fibroblasts were cultured on Type I collagen-coated silicon membranes and subjected to cyclic mechanical stimulation. The cells assumed a spindle shape, and the cytoskeleton components, including F-actin filaments and tubulin, were aligned perpendicular to the stretch force vector. Tyrosine-phosphorylated molecules which are localized in focal adhesion plaques and MAP kinase might be related to signal transduction caused by mechanical stress.2.When peripheral blood leukocytes/monocytes were cultured with M-CSF,the number of osteoclast like cells, which were multinuclear, lamellipodia-positive and TRAP-positive cells increased. When these cells were cultured on slices of bovine dental root, pit formations were observed. Macrophages of PBL proliferate by blastogenesis and a part of the blastgenic macrophages differentiate to osteoclasts by M-CSF and 1,25 (OH) _2D_3. Moreover the number of osteoclast-like cells shown in PBL … More treated in M-CSF or GM-CSF was suppressed by coculture fluid of PBL/PDL cells.3.Mechanical stress of tooth movement differently affected the alveolar bone and cellular cementum ; the bone was resorbed whereas the cementum resisted resorption and its calcification is inhibited under the compressive force of tooth movement. The expression of CH-6S is related to the compressive force in non-hyalinized and hyalinized PDL,whereas that of CH-4S/DS is not influenced by the mechanical stress. Osteocalcin expression of cementblasts forming acellular cementum is similar to that of cells forming cellular cementum as well as osteoblasts and odontblasts, and has a role for calcification of acellular cementum. Topical administration of a bisphosphonate (Risedronate) inhibits orthodontic tooth movement and root resorption.4.Localization and distribution of the ECMs ; Type I,II,III,X collagen, osteocalcin and GAGs were analyzed, using immunohistochemical techniques. The marked regional differences cxit in the phenotypic expression of ECMs in tissues, may reflect the local functional environment and cellular response. Less

1.在I型胶原蛋白涂层的硅膜D上培养了塑性细胞样细胞，MC3T3-EL或牙周 - 粘合物衍生成成纤维细胞，以使细胞刺激细胞。微管蛋白垂直于拉伸力量对齐。多光学，层状阳性和诱捕阳性细胞在牛牙根上的最终增加。 PDL细胞。3。牙齿运动受到影响的机械应力，牙槽骨和细胞固定性均抑制了钙化，并抑制了钙化的压缩力。类似于形成胶质的细胞以及成骨细胞和牙胶细胞，而odontblasts则在钙化骨质的钙化中起作用。在组织叔叔环境和细胞反应中，使用免疫组织化学技术分析了骨钙素和GAG