Molecular biological analysis of the mechanism of acute renal failure

急性肾衰竭机制的分子生物学分析

基本信息

批准号：
07457242
负责人：
TOMITA Kimio
金额：
$ 4.03万
依托单位：
Kumamoto University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
1995
资助国家：
日本
起止时间：
1995 至 1996
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07457242/
关键词：
acute renal failure gene glomerulus tubules endothelin receptor

项目摘要

We investigated the expression and regulation of endothelin-1 protein and mRNA,endothelin converting enzyme protein and mRNA,A type endothelin receptor mRNA,B type endothelin receptor mRNA in ischemic and cyclosporine-induced acute renal failure. Endothelin-1 mRNA started to increase from 30 minutes to several hours after ischemic insult or cyclosporine administration. A type endothelin receptor mRNA decreased in both types of acute renal failure. B type endothelin receptor mRNA increased in ischemic acute renal failure. However, it decreased in cyclosporine-induced acute renal failure. Endothelin converting enzyme protein and mRNA decreased in cyclosporine-induced acute renal failure. To elucidate the mechanism of inhibition of endothelin converting enzyme protein and mRNA,regulation of endothelin converting enzyme was investigated in cultured endothelial cells. Endothelin-1 has an inhibitory effect on endothelin converting enzyme protein and mRNA through B type endothelin receptor. These data suggests that impairments of renal function may be protected by the decrease in A type endothelin receptor and increase in B type endothelin receptor in ischemic acute renal failure. As for cyclosporine-induced acute renal failure, impairments of renal function may be protected by the decrease in A type endothelin receptor and decrease in endothelin converting enzyme through B type endothelin receptor.

我们研究了内皮素-1蛋白和mRNA的表达和调节，内皮素转化酶蛋白和mRNA，一种类型的内皮素受体mRNA，B型内皮素受体mRNA在缺血性和环孢菌素诱导的急性肾衰竭中。内皮素-1 mRNA在缺血性损伤或环孢菌素给药后的30分钟开始增加到几个小时。两种类型的急性肾功能衰竭的类型内皮素受体mRNA均降低。 B型内皮素受体mRNA在缺血性急性肾衰竭中增加。然而，它在环孢菌素诱导的急性肾衰竭中降低。内皮素转化酶蛋白和mRNA降低了环孢菌素诱导的急性肾衰竭。为了阐明抑制内皮素转化酶蛋白和mRNA的机制，在培养的内皮细胞中研究了内皮素转化酶的调节。内皮素-1通过B型内皮素受体转化酶蛋白和mRNA具有抑制作用。这些数据表明，肾功能的损害可能受到内皮蛋白受体的降低的保护，而在缺血性急性肾衰竭中，B型内皮素受体的增加。至于环孢菌素诱导的急性肾衰竭，肾功能的损伤可能受到类型内皮素受体的降低和内皮素通过B型内皮素受体转化酶的减少的保护。