Novel enteric nerve plexus links enteric endocrine system with gastrointestinal motility

新型肠神经丛将肠内分泌系统与胃肠运动联系起来

• 批准号: 07457004
• 负责人: TORIHASHI Shigeko
• 金额: $ 4.93万
• 依托单位: Nagoya University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07457004/
• 关键词: Gastrointestinal tract; Endocline system; Enteric nervous system; Pacemaker; c-kit; Sponteneous contraction; APC; Calcium binding protein; c-kit; 摘出平滑筋標本; 平滑筋自発性収縮能; イヌ近位結腸; 幽門近傍大弯側; モルモット結腸括約筋; モルモットOddi筋; 平滑筋ペースメーカー; 消化管運動; 消化管内分泌; 平滑筋

The main purpose of this research was as follows ;1) Identification of pacemaker cells of the spontaneous muscle contractions. 2) Analysis of the enteric nervous system which control spontaneous contractions. 3) Contractile response to the stimulation of the mucosa.We obtained four research results.1) Pacemaker cells were located at the submucosal surface of the colonic circular muscle layr in the dog, Guinea-pig and the mouse. They were a kind of special smooth muscle cells.2) Pacemaker cells expressed c-kit receptor tyrosine kinase immunoreactivity and their development depended on c-kit signaling pathway.3) Some enteric neurons expressed immunoreactivity of neurocalcin which is a neuuron specific calcium binding protein. Afferent nerve ending originated from vagus also expressed neurocalcin immunoreactivity4) The tumor suppressor gene APC was expressed on the mucosa of the colon. The products of APC were co-localized with beta catenin and with DLG.It may be involved in the proliferation and differentiation of mucosal epithelial cells.This research project was started by S.kobayashi as a head investigator from 1995 and after his death. S.Torihashi continued and completed.

本研究的主要目的如下：1）自发肌肉收缩起搏细胞的鉴定。 2）控制自发收缩的肠神经系统分析。 3)对粘膜刺激的收缩反应。我们获得了四项研究结果。1)起搏细胞位于狗、豚鼠和小鼠结肠环肌层的粘膜下表面。它们是一种特殊的平滑肌细胞。2）起搏细胞表达c-kit受体酪氨酸激酶免疫反应性，其发育依赖于c-kit信号通路。3）一些肠神经元表达神经钙蛋白的免疫反应性，神经钙蛋白是一种神经元特异性钙结合蛋白。起源于迷走神经的传入神经末梢也表达神经钙素免疫反应性4)抑癌基因APC在结肠粘膜上表达。 APC的产物与β连环蛋白和DLG共定位。它可能参与粘膜上皮细胞的增殖和分化。该研究项目是由S.kobayashi作为首席研究员从1995年起及其去世后开始的。 S.Torihashi 继续并完成。

项目成果

Watanabe Y,Ito S,Harada T,Ando H,Seo T,Kobayashi S,Ozaki T: "Unique spindle cells in the involved ileal segment of rats with congenital aganglionosis." J Pediat Surg. 32. 1229-1233 (1997)

Watanabe Y、Ito S、Harada T、Ando H、Seo T、Kobayashi S、Ozaki T：“先天性无神经节细胞病大鼠受累回肠段中的独特梭形细胞。”

Torihashi S: "Expression of nitric oxide syntase in mucosal cells" Histochem Cell Biol. 105. 33-41 (1996)

Torihashi S：“粘膜细胞中一氧化氮合酶的表达”组织化学细胞生物学。

Kobayashi S: "The centenary of the problem of the interstitial cells" Acta Anat Nipppn. 71. 629-637 (1996)

小林 S：“间质细胞问题一百周年”Acta Anat Nipppn。

Kobayashi S: "A smooth muscle nodule producing 10-12 cycle/min" Arch Histol Cytol. 59. 159-168 (1996)

Kobayashi S：“产生 10-12 周期/分钟的平滑肌结节”Arch Histol Cytol。

Nishi K: "Gastrointestinal function.Regulation and disturbances," Kasuya Y.,Tsuchiya M.,Matsuo Y (Eds), 27-43 (1996)

Nishi K：“胃肠功能。调节和干扰”，Kasuya Y.，Tsuchiya M.，Matsuo Y（编辑），27-43（1996）