Imaging of dynamic structure of molecular and cellura organization by ultramicroscopic tequniques.

通过超显微技术对分子和细胞组织的动态结构进行成像。

基本信息

批准号：
07308070
负责人：
USUKURA Jiro
金额：
$ 3.2万
依托单位：
Nagoya University School of Medicine
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (A)
财政年份：
1995
资助国家：
日本
起止时间：
1995 至 1996
项目状态：
已结题

项目摘要

Purpose of this project is to reveal the three dimensional dynamic structure of cytoskeleton or membrane skeleton related to the signal transduction. For this purpose, we are developing new imaging technology including atomic force microscope, energy filtering electron microscope and other improved video-microscope. Furthermore, this project is also dedicated a little to disclose the molecular structure of proteins, since signal transduction is based on molecular recognition of each related proteins. In other words, a series of molecular recognition is a cascade of signal transduction. Obviously, molecular-molecular recognition is involved in the shape or surface structure of molecules.Activity of individuals in this team.Project leader, Usukura made a report of this project, at the same time, he tried to image the transcription of DNA in this year. Dr.Sokabe revealed relationship between opening of mechanosensory channels and cytoskeletons by patch clump method combined with video-enhanced microscopy. Dr.Kusumi reported regulatory system of membrane proteins movement relating to the domain structure comprised of cytoskeletons beneath the membrane. Dr.Ishikawa revealed undercoat structure of the plasma membrane using confocal microscope. Dr.Kinoshita succeeded to observe actin filaments movement and interaction with the membrane in living state using new type of nanosecond multi-imaging microscope. Dr.Katayma and Dr.Toyoshima described molecular structures of actin filaments by mica flake method and ice embedding electron microscopic methods respectively. Drs.Owaribe and Tsukita presented molecular biological data of action binding proteins.

该项目的目的是揭示与信号转导相关的细胞骨架或膜骨架的三维动态结构。为此，我们正在开发新的成像技术，包括原子力显微镜、能量过滤电子显微镜和其他改进的视频显微镜。此外，该项目还致力于揭示蛋白质的分子结构，因为信号转导是基于每个相关蛋白质的分子识别。换句话说，一系列的分子识别是信号转导的级联。显然，分子间的识别涉及到分子的形状或表面结构。这个团队中个体的活动。项目负责人薄仓做了这个项目的报告，同时，他试图对这个项目中DNA的转录进行成像。年。 Sokabe博士通过膜片丛方法结合视频增强显微镜揭示了机械感觉通道的开放与细胞骨架之间的关系。 Kusumi博士报告了与膜下细胞骨架组成的域结构相关的膜蛋白运动调节系统。 Ishikawa博士使用共焦显微镜揭示了质膜的底涂层结构。 Kinoshita博士利用新型纳秒多重成像显微镜成功地观察了活体状态下肌动蛋白丝的运动以及与膜的相互作用。 Katayma博士和Toyoshima博士分别用云母片法和冰包埋电子显微镜方法描述了肌动蛋白丝的分子结构。 Owaribe 博士和 Tsukita 博士展示了作用结合蛋白的分子生物学数据。