Epidemiological studies on dietary habit and daily activity in the aged and study on anti-aging factors by using cellular immunity as an index

老年人饮食习惯、日常活动的流行病学研究及以细胞免疫为指标的抗衰老因素研究

基本信息

批准号：
06670419
负责人：
MORIGUCHI Satoru
金额：
$ 1.34万
依托单位：
The University of Tokushima
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1994
资助国家：
日本
起止时间：
1994 至 1996
项目状态：
已结题

项目摘要

1.T cell differentiation and maturation in thymus and vitamin E :Six weeks old, F344 rats were fed control or high vitamin E diet, which contained 10 times higher vitamin E (alpha-tocopherol nictonate) than control diet, for 7 weeks. Rats from the high vitamin E group showed the increases in the proportions of both CD4+CD8- and CD4-CD8+ T cells in their thymocytes. In adition, the increase of IL2 production and the decrease of PGE_2 were shown in thymocytes of rats fed the high vitamin E diet. Thymic epithelial cell (TEC) function was also increased by feeding of the high vitamin E diet. Especially, the binding of immature T cells to TEC was significantly increased by the high vitamin E diet, which was associated with the increased expression of ICAM.However, vitamin E had no effect on macrophage function (Negative selection) existing between thymic cortex and medulla. These results suggest that high vitamin E diet enhances T cell differentiation and maturation in thymus via both the increased TEC function (Positive selection) in thymic cortex and increased proliferation of muture T cells in thymic medulla.2.The decrease of cellular immunity with aging, vitamin E and exercise training :F344 rats were fed control or the high vitamin E diet for 12 months from young age (8 weeks old). Then, their splenocyte function (mitogenesis) was investigated. As a result, rats fed control diet showed the decreased proliferation of splenocytes with Con A or PHA.On the other hand, rats fed the high vitamin E diet showed a significant increase of splenocyte proliferation, which was almost the same as that of young rats (8 weeks old). This was associated with both the increase of splenic macrophage function and the increased susceptibility of splenic lymphocytes to mitogen by feeding high vitamin E diet. Furthermore, the decrease of cellular immunity with aging was also improved by exercise training.

1.胸腺和维生素E的T细胞分化和成熟：六周大，F344大鼠接受对照或高维生素E饮食，其含有比对照饮食高10倍的维生素E（α-托酚含量），持续7周。来自高维生素E组的大鼠显示其胸腺细胞中CD4+ CD8-和CD4-CD8+ T细胞的比例增加。在ADITION中，在喂养高维生素E饮食的大鼠胸腺细胞中显示了IL2产生的增加和PGE_2的降低。胸腺上皮细胞（TEC）功能也通过喂养高维生素E饮食提高。特别是，高维生素E饮食与ICAM的表达相关的高维生素E饮食显着增加了未成熟T细胞与TEC的结合。但是，维生素E对胸腺皮质和髓质之间存在的巨噬细胞功能没有影响。这些结果表明，高维生素E饮食通过提高胸腺皮质中TEC功能（阳性选择）增强了T细胞的分化和成熟。然后，研究了它们的脾脏功能（有丝分裂）。结果，喂养控制饮食的大鼠伴有con a或pha的脾细胞的增殖减少，另一只喂食高维生素E饮食的大鼠显示出脾脏增殖的显着增加，这与年轻大鼠（8周大）几乎相同。这与脾巨噬细胞功能的增加以及通过喂养高维生素E饮食的脾淋巴细胞对有丝分裂原的敏感性的增加有关。此外，通过运动训练，还可以改善细胞免疫的降低。