Study on regulation of placental functions : Involvement of ovarian factors in the mechanism.

胎盘功能调节研究：卵巢因素参与的机制。

基本信息

批准号：
06454113
负责人：
SHIOTA Kunio
金额：
$ 4.67万
依托单位：
The University of Tokyo
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
1994
资助国家：
日本
起止时间：
1994 至 1996
项目状态：
已结题

项目摘要

The placenta plays an essential role in fetal growth and survival. maintenance of maternal metabolic status during pregnancy by virtue of its multiple functions relating to the transport of nutrients and waste between mother and tetus, moduiating of the matemal and fetal immune systems to prevent rejection of each other, and its endocrine function of maintaining the pregnancy. Successful development of errbryo is totally dependent on placental function. Considering these multiple functions, not only functions that are required constantly but also pregnancy stage specific events such as placental lactogen synthesis, the placenta is thought to express an abundance of biologically active molecules. In the series of experiments, we have analyzed and identified the almost 800 proteins which are stage-specifically expressed on the placental plasma membrane of the rat placenta. We have also cloned the cDNAs encoding novel prolactin (PPL) like molecules, PL-lm and PLP-D,in the rat placenta. PL-lm RNA is specitically expressed at mid-pregnancy, and PLP-D is specific to late pregnancy. In addition to these placental PPL-like molecules, we found GD3 and GM3 as the main gangliosides in the placenta. The composition changes stage specific manner in the rat placenta. Using prolonged pregnancy models and the various stage-specific indexes as mentioned above, it became clear that the stage-specific functions of placenta are controlled by matemal ovarian factors. Expression of these stage-specific functions of the placenta is related to the differentiation of trophoblasts and changes in the cellular composition. Furthermore, we got the evidence indicating that methylation of DNA of CpG islands or in the promoter regions is involved in the placental specific gene-expression and differentiation of the placenta.

胎盘在胎儿生长和生存中起着至关重要的作用。孕产妇在怀孕期间的维持，凭借其多种功能与母亲和tetus之间的养分和废物的运输有关，对玛格梅尔和胎儿免疫系统的调节以防止彼此排斥以及维持妊娠的内分泌功能。 Errbryo的成功发展完全取决于胎盘功能。考虑到这些多种功能，不仅需要不断需要的功能，而且还需要妊娠期特定事件（例如胎盘乳元合成），胎盘被认为表达了丰富的生物活性分子。在一系列实验中，我们分析并鉴定了几乎800个蛋白质，这些蛋白在大鼠胎盘的胎盘质膜上特异性表达。我们还克隆了大鼠胎盘中编码新型催乳素（PPL）（例如分子PL-LM和PLP-D）的cDNA。 PL-LM RNA在怀孕中期表达，PLP-D特定于妊娠晚期。除了这些胎盘样分子外，我们还发现了GD3和GM3是胎盘中的主要神经脂情况。组成改变了大鼠胎盘中的特定阶段。如上所述，使用延长的怀孕模型和各个阶段特异性指数，很明显胎盘的特定阶段功能受到马结构卵巢因子的控制。胎盘的这些阶段特异性功能的表达与滋养细胞的分化和细胞组成的变化有关。此外，我们得到的证据表明，CpG岛或启动子区域中DNA的甲基化参与了胎盘特异性基因表达和胎盘的分化。