Joint Study of Immunomodulation for Corneal Graft Survival

角膜移植物存活免疫调节联合研究

• 批准号: 05045051
• 负责人: ISHIKAWA Satoshi
• 金额: $ 3.84万
• 依托单位: School of Medicine, Kitasato University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for international Scientific Research
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-05045051/
• 关键词: Corneal grafting; Pig; Inbred rat; Ultraviolet; Monochromator; 紫外線; ブタ; 大動物実験

We have used Monochromator (CT-25 IV,Jasco) for corneal graft surviving and choose specific narrow band for irradiation with the support of Mr.Keith Langmack in Medical Physics, Addenbrooke's Hospital, Cambridge. Dr.Atsushi Minamoto, Dept.of Ophthalmology, Hiroshima University who was studying Transplantation Immunology under the supervision of Mr.Watson helped for corneal grafting and post-operative e observation. We have finally detected the specific band and the minimum dose for corneal graft surviving (300nm, 200mJ/cm^2).Dr.Katami had visited to Cambridge in November to start the pig corneal grafting using the Monochromator. However, in the same condition, the effect of ultraviolet to the pig cornea was not so significant than that the rat for long-term corneal graft surviving. Dr.William B.Amos, Molecular Biology, MRC,Cambridge helped us to investigate inability of ultraviolet effect in pig corneal grafting. With the powerful instrument of confocal microscope which can show three … More dimensional microscopic image of the object, we understood that pig endothelial cells were damaged so that the graft itself could not support for maintain ace of the cornea. This indicates that dose of ultraviolet to the pig cornea should be lower that that of rat cornea for graft survival. We have summarized the experiments as follows.1.Inbred rat work--- This is quite useful model for detect specific wave length and total dose of ultraviolet for corneal graft surviving.2.Pig work---The work needs more efforts to detect safety dose of ultraviolet for corneal graft surviving. Confocal microscopic examination is necessarily for confirmation of safety level of ultraviolet.3.Clinical trial--- The method we used in pig would be useful when we would try to utilize ultraviolet for clinical corneal transplantation. Mr.Malcolm Kurr-Muir, cornea specialist is responsible for pilot study in Dept.of Ophthalmology, University of Cambridge. After the pilot study confirmed without side effect, we will expand the clinical trial in different institutes in the world. Less

我们已经使用了单色仪（CT-25 IV，Jasco）进行角膜移植物存活，并在剑桥的Addenbrooke医院的医学物理学先生的支持下，在Keith Langmack先生的支持下选择特定的窄带进行照射。海洛岛大学眼科部门的Atsushi Minamoto博士，在Watson先生的监督下研究移植免疫学，帮助角膜移植和术后E观察。我们最终检测到了角膜移植物存活率的特定带和最小剂量（300nm，200mj/cm^2）。然而，在相同的条件下，紫外线对猪角膜的影响并不比长期角膜移植物生存的大鼠的影响。使用强大的共聚焦显微镜仪器，可以显示三个……更尺寸的显微镜图像，我们知道猪内皮细胞受损，因此移植物本身无法支撑维持角膜的王牌。这表明紫外线对猪角膜的剂量应低于移植物存活的大鼠角膜的剂量。 We have summarized the experiments as follows.1.Inbred rat work--- This is quite useful model for detect specific wave length and total dose of ultraviolet for corneal graft survival.2.Pig work---The work needs more effort to detect safety dose of ultraviolet for corneal graft survival.Confocal microscopic examination is necessary for confirmation of safety level of ultraviolet.3.Clinical trial--- The method we used in pig would be useful当我们尝试利用紫外线进行临床角膜移植时。角膜专家Malcolm Kurr-Muir先生负责在剑桥大学眼科部的试点研究。试点研究证实没有副作用后，我们将扩大世界上不同机构的临床试验。较少的

项目成果

M.Katami,P.W.Madden,D.J.G.White,P.G.Watson: "The extent of immunological privilege of orthotopic corneal grafts in the inbred rat." Transplantation.48. 371-376 (1989)

M.Katami、P.W.Madden、D.J.G.White、P.G.Watson：“近交系大鼠原位角膜移植物的免疫特权程度。”

M.Katami.: "Is a corneal transplantation immunologically privileged from graft rejection?" Annals Academy of Medicine Singapore.20. 432-438 (1991)

M.Katami.：“角膜移植在免疫学上是否能免受移植物排斥？”

M.Katami,D.J.G.White and P.G.Watson.: "Site-specific immunosuppression for corneal graft survival in the rat." Transplant.Proc.24. 1406-1408 (1992)

M.Katami、D.J.G.White 和 P.G.Watson.：“位点特异性免疫抑制可促进大鼠角膜移植物的存活。”