Development of Autonomous Active Micro-Biosensor using Micromachine Technology

利用微机械技术开发自主主动微型生物传感器

基本信息

批准号：
05555116
负责人：
IGARASHI Isemi
金额：
$ 6.14万
依托单位：
Toyota Physical and Chemical Research Institute
依托单位国家：
日本
项目类别：
Grant-in-Aid for Developmental Scientific Research (B)
财政年份：
1993
资助国家：
日本
起止时间：
1993 至 1995
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-05555116/
关键词：
Micro Gulucose Sensor Autonomous Active Sensor Micro Actuator Medical Applications of Micromachine Technology Medical Application of Ultrasonic Micro-cleaning System Platinum Black Sensor マイクロマシンの医用応用グルコースセンサ自律センサ超音波洗浄生体埋込み型センサ

项目摘要

The key point of the development of the implantable artificial pancreas is that of a small implantable glucose sensor which can measure blood glucose levels directly for a long time. We have proposed an in situ ultrasonic cleaning method to remove the adhered materials on the sensor surface from blood or body fluid. We have developed a micro glucose sensor fabricated in a needle using a newly developed electrodeposition method of Pt black with physical stability. Pt black was electrodeposited on a Pt write (0.127 mmphi) sealed in a 23-G needle (ID = 0.45 mmphi) under irradiation of ultrasonic wave (38 kHz, 30 W) from a conventional Pt-electroplating solution for 80 s at -80 mV vs.Ag-AgCl. Pt black tightly adhered on the surface of a Pt wire, while that deposited without the irradiation of ultrasonic wave was easily removed from the surface by sonication. GOD was absorbed to the Pt black surface from 0.75 ml of 5300 U/ml solution (pH 7.45) at 6ﾟC for 30 min and air-dried. GOD was fixed with glutaraldehyde and then with Nafion solution at 6ﾟC.The activity of GOD immobilized on the Pt black was found to be 5.5 mU/sensor on the first day and it gradually decreased with time but kept more than 74% of the initial value after 26 days storing in PBSS at 6ﾟC.The sensor responds to the stepwise change of glucose concentrations up to about 50 mg/dl within a few minutes and the output currents varied with oxygen partial pressures.

植入式人工胰腺开发的关键是能够长期直接测量血糖水平的小型植入式血糖传感器，我们提出了一种原位超声波清洗方法，去除传感器表面的粘附材料。我们开发了一种在针中制造的微型葡萄糖传感器，采用新开发的铂黑电沉积方法，将具有物理稳定性的铂黑电极沉积在密封的铂写入（0.127 mmphi）上。 23-G 针（ID = 0.45 mmphi）在传统 Pt 电镀溶液的超声波（38 kHz，30 W）照射下在 -80 mV 下照射 80 秒，而 Pt 黑紧紧粘附在表面。铂丝，而没有超声波照射的沉积物很容易通过超声处理从表面去除。将 0.75 ml 5300 U/ml 溶液 (pH 7.45) 在 6°C 下固定 30 分钟并风干 GOD，然后用戊二醛固定，然后用 Nafion 溶液在 6°C 固定。第一天发现Pt black为5.5 mU/传感器，随着时间的推移逐渐下降，但保持在超过在 6°C 的 PBSS 中储存 26 天后，达到初始值的 74%。传感器在几分钟内响应葡萄糖浓度的逐步变化，最高可达约 50 mg/dl，输出电流随氧分压变化。