STUDIES ON DYNAMIC PROPERITIES OF CYTOSKELETAL FILAMENT SYSTEMS

细胞骨架丝系统的动力学特性研究

• 批准号: 04304057
• 负责人: MABUCHI Issei
• 金额: $ 8.32万
• 依托单位: UNIVERSITY OF TOKYO
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Co-operative Research (A)
• 财政年份: 1992
• 资助国家: 日本
• 起止时间: 1992 至 1993
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-04304057/
• 关键词: Cytokinesis; Action filaments; Microtubules; Intermediate filaments; Contractile ring; Mitotic apparatus; Dynein; Kinesin; 細胞接着装置; ミオシン; リン酸化; Cytokinesis; Action filaments; Microtubules; Intermediate filaments; Contractile ring; Mitotic apparatus; Dynein; Kinesin; 細胞接着装置; ミオシン; リン酸化

1) Cytokinesis : It has been shown that both protein phosphorylation and a low molecular weight protein rho are involved in the organization of the contractile ring. 2) In vitro motility : Tropomyosin-troponin enhances efficiency of chemical-mechanical energy transfomation in the actomyosin interaction. Kinesin head domain has been shown to be able to slide microtubules (MTs). 3) Structure-function relationship of actin : Chimera actins have been produced from Tetrahymena and Dictyostelium. A segment spanning from res. 38-83 in the actin sequence has been shown to interact with DNase I.4) Membrance-skeleton interaction : ERM family proteins are involved in constitution of the actin cytoskeleton. These proteins directly interact with the membrane protein CD44.5) Mitotic apparatus : Hexylene glycol, a MT-stabilizer, enlarges metaphase spindle at concentrations less than 3%, and slows chromosome movements. 6) Motility in chromatophores : Ca ion concentration raises prior to aggregation of pigments in melanophores. Pigments moves through interaction between motor proteins and MTs. 7) Brain MTs : An epitope for anti-tubulin antibody E3B8 is present in res. 432-452 which is common in all alpha-tubulins. 8) Dynamic properties of MTs : MTs switch from depolymerization phase to polymerization phase at the site of MAPs binding. The MT-satsbilizing ability of MAPs is abolished by phosphorylation by MPF.22S dynein binds to MTs in an ATP-sensitive manner and thereby stabilizes the MTs. 9) Intermediate filaments : Type 2A phosphatase associates with neurofilaments in the spinal cord and stabilizes the neurofilaments. It ia likely that CaM-kinase II is a intermediate filament-kinase during cell division.

1）细胞因子：已经表明，蛋白质磷酸化和低分子量蛋白Rho都参与收缩环的组织。 2）体外运动：肌球蛋白 - 肌钙蛋白提高了肌动蛋白相互作用中化学机械能传输的效率。驱动蛋白头域已被证明能够滑动微管（MTS）。 3）肌动蛋白的结构功能关系：嵌合肌动蛋白是由四膜膜和柱状固有产生的。从RES跨越的段。肌动蛋白序列中的38-83已显示与DNase I.4）膜骨骼相互作用相互作用：ERM家族蛋白参与肌动蛋白细胞骨架的构成。这些蛋白直接与膜蛋白CD44.5）有丝分裂设备相互作用：己二醇，一种MT稳定剂，以低于3％的浓度增强中期纺锤体，并减慢染色体运动。 6）色谱中的运动性：在黑素化物中凝聚之前，CA离子浓度提高。色素通过运动蛋白和MT之间的相互作用移动。 7）脑MTS：Res中存在抗微管蛋白抗体E3B8的表位。 432-452在所有α-微管蛋白中都是常见的。 8）MTS的动态特性：MTS从地图结合的位点上从解聚阶段转变为聚合阶段。 MPF.22S Dynein以ATP敏感的方式与MT结合，从而消除了MAP的MT - 胃屈服能力，从而稳定了MT。 9）中间丝：2A型磷酸酶与脊髓中的神经丝相关并稳定神经丝。凸轮激酶II可能是细胞分裂过程中的中间丝激酶。