Study of Free Radicals in Tissue and Transdermal Drug Delivery by using in vivo ESR with Spectral-Spatial ESR-CT Imaging

利用体内 ESR 和光谱空间 ESR-CT 成像研究组织和透皮药物递送中的自由基

基本信息

批准号：
03671032
负责人：
UTSUMI Hideo
金额：
$ 1.34万
依托单位：
Showa University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1991
资助国家：
日本
起止时间：
1991 至 1993
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-03671032/
关键词：
ESR Imaging Free Radical Active Oxygens CT CT イメージングスペクトル逆投影法反復法ラジカル

项目摘要

The several techniques for ESR imaging have been developed. The conventional methods for ESR-CT are based on the assumption that only one spectralcomponetn is existing in all space observed. No image can be constructed if more than two signal componets are coexisting in different part. In order to accomplish the image construction from plural signal components, we tried to connect spectral-spatial imaging technique with CT imaging.1. We report the ESR-CT imaging by the phantom involving two radical species giving different signals. Spectral-spatial images obtained on four derections in X-Z plane. The spatial information was separated from the spectral-spatial images by each radical component. Two separation methods were compared. In one method, separation could not be complete. The border was broadened in other method. Two sets of CT images of two signal components were reconstructured. CT images have good agreement with arrangement of phantom.2. The phantom involving liposomes which encapsulated nitroxide (150 mM) and free nitroxide (3.3mM) was made. We obtained the ESR-CT imaging by L-band ESR.CT images have good agreement with the distribution of loposomes and free nitroxide.These results demonstrate the possibility of ESR-CT for the sample including plural signal components.

已经开发了几种 ESR 成像技术。 ESR-CT 的传统方法基于这样的假设：在所有观测到的空间中仅存在一种光谱成分。如果两个以上的信号分量共存于不同部分，则无法构建图像。为了实现多信号分量的图像构建，我们尝试将光谱空间成像技术与CT成像相结合。 1．我们报告了模型的 ESR-CT 成像，涉及两种发出不同信号的自由基物质。在 X-Z 平面的四个方向上获得的光谱空间图像。空间信息通过每个自由基成分从光谱空间图像中分离出来。比较了两种分离方法。在一种方法中，分离不能完全。用其他方法拓宽了边界。重建了两个信号分量的两组 CT 图像。 CT图像与体模排列吻合较好。2．制作涉及封装硝基氧(150mM)和游离硝基氧(3.3mM)的脂质体的体模。我们通过L波段ESR获得了ESR-CT成像。CT图像与脂质体和游离硝基氧的分布具有很好的一致性。这些结果证明了ESR-CT对包含多个信号分量的样品的可能性。