Study of muscle and bone tissue using tail suspended rat

悬尾大鼠肌肉和骨组织的研究

基本信息

批准号：
03670018
负责人：
KOSUGI Kazuo
金额：
$ 1.28万
依托单位：
Jikei University, School of Medicine,Lecturer
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1991
资助国家：
日本
起止时间：
1991 至 1992
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-03670018/
关键词：
Tail suspension Atrophy of muscle M. tibialis anterior M.gastrocnemius M. plantaris Blood biochemical substance Serum albumin Microgravity 筋組織骨組織萎縮 Tail Suspension 血中蛋白質血中脂質赤筋線維白筋線維

项目摘要

An atrophic phase of muscles was studied by using TS rat, suspended from the ceiling of cage. After 10 weeks, blood was taken under ether anaesthesia for a biochemical measurement. They were serum albumin, total cholesterol, urea nitrogen, creatinine, uric acid, CK, GOT, GPT and LDH. After that, some viscerals and muscles were removed and weighed. Some muscles were sectioned and stained by sudan black B for microscopic observation. Area of red and white muscle fibers was measured by automatic planimeter, which was connected to a computer.The level of serum albumin and wet liver weight were significantly low in the TS group. It suggests the lowering of albumin, made in the liver, by 10weeks tail suspension. On the other side, the influence to muscle was calculated in next expression, i. e, (TS-C)/C X 100. The influenced degree was shown on the muscle weight, i. e, 12.7% in the tibialis anterior, 26.4% in the gastrocnemius and 23.4% in the plantaris. Those on the area of fibers were 6.6% of white and 3.7% of red fibers in the tibialis anterior, 23.7% of white and 17.9% of red in the gastrocnemius, 27.7% of white and 33.5% of red in the plantaris. These results suggest that the tail suspension strongly effects to the lower-limb flexors.Considering other research articles, it will need to analyze various conditions. For example, microgravity, nutrients and exercise should be studied, in addition to the differences of age in experimental animals, or in each situated muscle.

通过使用TS大鼠研究了从笼子的天花板上悬浮的肌肉萎缩阶段。 10周后，在以太麻醉下进行血液进行生化测量。它们是血清白蛋白，总胆固醇，尿素氮，肌酐，尿酸，CK，GOT，GPT和LDH。之后，除去一些内脏和肌肉并称重。一些肌肉被苏丹黑B截切和染色，以进行微观观察。红色和白色肌肉纤维的面积通过自动平面测量，该面积连接到计算机。TS组的血清白蛋白和湿肝脏重量显着较低。它表明，通过10周的尾巴悬架，在肝脏中制造的白蛋白降低。另一方面，对肌肉的影响是在下一个表达式中计算出来的。 e，（ts-c）/c x 100。受影响的程度显示在肌肉重量上，i。 E，胫骨前部为12.7％，甲壳虫的26.4％，plantaris的23.4％。纤维区域的白色为6.6％，胫骨前的红色纤维的3.7％，白色的23.7％，甲壳虫的红色为17.9％，白色的27.7％，plantaris中的红色和33.5％的红色。这些结果表明，尾部悬浮液对下限的屈肌有很大影响。考虑到其他研究文章，它将需要分析各种条件。例如，除实验动物的年龄差异外，还应研究微重力，营养和运动。