Effects of obioactin and obiopeptide-1, as a biological response modifier (BRM), on murine macrophage functions

obiactin 和 obiopeptide-1 作为生物反应调节剂 (BRM) 对小鼠巨噬细胞功能的影响

基本信息

批准号：
03660320
负责人：
KONO Michiko
金额：
$ 1.54万
依托单位：
The University of Tokyo
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1991
资助国家：
日本
起止时间：
1991 至 1992
项目状态：
已结题

项目摘要

Effects of obioactin and obiopeptide-1, as a biological response modifier, obtained from hyperimmuned bovine serum against toxoplasma gondii, on murine macrophage functions were examined. The results obtained are as follows;1) in vitro study: The changes of glucose consumption, oxygen intermediate release, ruffle formation, chemotactic responses, and toxoplasmacidal activity in murine macrophages cultivated with obioactin and obiopeptide-1 were examined. No difference was observed in phagocytic activity and Ia-positive cell numbers of murine macrophages caltivated with or without these 2 peptides. However, a significant increase of glucose consumption, oxygen intermediate release, and toxoplasmacidal activity in murine macrophage caltivated with these 2 peptides. The migration of murine macrophages caltivated with these 2 peptides was also observed at a dose related manner. The ruffle formation of macrophages caltivated with these 2 peptides were higher than those in macrophages caltivated with medium alone. These results indicated that obioactin and obiopeptide-1 have a macrophage activating factors and shows the character of biological response modifier.2) in vivo study: The changes of the lysosomal enzyme activities(acid phosphatase, beta-N-acetyl- glucronidase, beta-glucronidase) in peritoneal and splenic macrophages of mice treated with these 2 peptides were examined. The lysosomal enzyme activities in both peritoneal and splenic macrophages was higher than those in macrophages from control mice. The protective activity against opportunistic infection with bacteri was also examined in cyclophosphamide- or carrageenan-pretreated mice. These peptides showed a protective activity against some bacterial infections.

研究人员检测了从针对弓形虫的超免疫牛血清中获得的 obiactin 和 obiopeptide-1（作为生物反应调节剂）对小鼠巨噬细胞功能的影响。所得结果如下： 1)体外研究：观察obioactin和obiopeptide-1培养的小鼠巨噬细胞的葡萄糖消耗、氧中间体释放、皱褶形成、趋化反应和弓形虫活性的变化。使用或不使用这 2 种肽培养的小鼠巨噬细胞的吞噬活性和 Ia 阳性细胞数量没有观察到差异。然而，用这 2 种肽培养的小鼠巨噬细胞中的葡萄糖消耗、氧中间体释放和弓形虫活性显着增加。还以剂量相关的方式观察到用这两种肽培养的鼠巨噬细胞的迁移。用这两种肽培养的巨噬细胞的褶皱形成高于仅用培养基培养的巨噬细胞。这些结果表明，obiactin和obiopeptide-1具有巨噬细胞激活因子，显示出生物反应调节剂的特性。2）体内研究：溶酶体酶活性（酸性磷酸酶、β-N-乙酰-葡萄糖醛酸酶、β-对用这 2 种肽处理的小鼠的腹膜和脾巨噬细胞中的葡萄糖醛酸酶）进行了检查。腹膜和脾巨噬细胞中的溶酶体酶活性均高于对照小鼠巨噬细胞中的溶酶体酶活性。还在环磷酰胺或角叉菜胶预处理的小鼠中检查了针对细菌机会性感染的保护活性。这些肽表现出针对某些细菌感染的保护活性。