Molecular Biological Approach to Nephritogenic Proteins

肾炎蛋白的分子生物学方法

基本信息

项目摘要

Fibronectin is an extracellular adhesive protein which actively functions during wound healing, cell migration, phagocytosis of Kupffer's cell or macrophages, etc. Cellular fibronectin promotes the differentiation of the neural crest cells to adrenergic phenotype of cells. Whereas the myotube formation process induced by the fusion of nyoblast was inhibited by fibronectin. This activity indicated that fibronectin is a differentiation inhibition substance. In the case of chondrocyte cell culture, fibronectin causes dedifferentiation of chondrocytes to a phenotype resembling the nesenchynal cell. Thus the fibronectin has inconsistent functions in the molecule. We thought from these published results that fibronectin could be a controlling morphology protein rather than a differentiation or dedifferentiation substance. Our target was to clone controlling morphology proteins, then we had to exclude many metabolic enzymes distributed in the organs(kidney, liver, lung etc.). We investigated … More to obtain or to discover proteins which change morphology, acting outside or beneath the membrane, through nephritis and fibrosis. We used antibodies against kidney basement membranes to obtain clones carrying cell surface vicinal proteins. Several inserts cDNAs obtained by library screenings of lambda-gtll were used as probes for Northern blot hybridization. The probe purified from 21st clone(lambda NH21)showed an increased level of expression through wound-healing/fibrosis. Sequenced NH21 was homologous to human profilin cDNA. Our nobel approach decreased the obtaining possibility of cytoplasmic metabolic enzyme eDNAs, and at the same time, this gave us to identity controlling morphology proteins speedily and to find out a protein links to signal transduction, that is, one of the obtained clones carried profilin cDNA.The approach using pentapeptide(VIO)which is a part of type III repeat of fibronectin, VIO induced thymocyte differentiation.We have not yet succeeded by the approach to obtain cDNA coding Nephritogenoside using PCR technique and direct hybridization of synthetic oligos to lambda-gtllcDNA library. Less
纤连蛋白是一种细胞外粘附蛋白,在伤口愈合,细胞迁移,kupffer细胞或巨噬细胞的吞噬作用等过程中起作用。细胞纤维蛋白会促进神经元细胞对细胞肾上腺素型的分化。而纤连蛋白抑制了由Nyoblast融合引起的肌管形成过程。该活性表明纤连蛋白是一种分化抑制物质。在软骨细胞培养物的情况下,纤连蛋白会导致软骨细胞的去分化为类似于Nesenchynal细胞的表型。纤连蛋白在分子中具有不一致的功能。我们认为,从这些发表的结果中,纤连蛋白可能是控制形态蛋白的一种,而不是分化或去分化物质。我们的目标是克隆控制形态蛋白,然后我们必须排除分布在器官(肾脏,肝,肺等)中的许多代谢酶。我们研究了……更多地获得或发现通过肾炎和纤维化改变形态,作用在膜外或下方的蛋白质。我们使用针对肾脏基底膜的抗体获得携带细胞表面替代蛋白的克隆。通过Lambda-GTLL的库筛选获得的几个插入物cDNA用作北印迹杂交的探针。从第21个克隆(Lambda NH21)纯化该探针通过伤口愈合/纤维化显示表达水平升高。测序的NH21与人类profilin cDNA同源。我们的诺贝尔方法降低了细胞质代谢酶EDNA的获得潜力,与此同时,这使我们迅速控制了身份,可以迅速控制形态蛋白质,并找出与信号转导的蛋白质联系,即使用p肽肽(vio)III(vio)III,这是一个cdna。胸腺细胞的分化。我们尚未通过使用PCR技术获得cDNA编码肾op的方法,并直接杂交合成寡聚与lambda-gtllcdna库。较少的

项目成果

期刊论文数量(8)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
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Hatsumi Taniguchi,Shigeo Kubomura,Hideyasu Hirano,Kumiko Mizue,,Midori Ogawa and Yasuo Mizugushi: "Cloning and characterization of a gene encoding a new thermostable hemolysin from Vibrio parahaemolyticus" FEMS Microbiology Letters. 67. 339-346 (1990)
Hatsumi Taniguchi、Shigeo Kubomura、Hideyasu Hirano、Kumiko Mizue、Midori Okawa 和 Yasuo Mizugushi:“编码副溶血弧菌新型热稳定溶血素的基因的克隆和表征”FEMS 微生物学快报。
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Keiroh Utsumi,Motoyuki Sawada Seiji Narumiya,Jun Nagamine et al Hideyasu Hirano,Hiromi Fujiwara: "Adhesion of immature thymocytes to thymic stromal cells through fibronectin molecules and its significance for the induction of thymocyte differentiation" Pr
Keiroh Utsumi、Motoyuki Sawada Seiji Narumiya、Jun Nagamine 等 Hideyasu Hirano、Hiromi Fujiwara:“未成熟胸腺细胞通过纤连蛋白分子粘附到胸腺基质细胞及其对诱导胸腺细胞分化的意义”Pr
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Hayato Nakamura, Kenzo Yamashiro, Kiyotaka Shibata, Sadao Gotoh, Kenji Hiranuma, Ken Higashi and Hideyasu Hirano: "Expression Levels of Fibrogenic Proteins in CCl_4 Treated Rat Liver"
Hayato Nakamura、Kenzo Yamashiro、Kiyotaka Shibata、Sadao Gotoh、Kenji Hiranuma、Ken Higashi 和 Hideyasu Hirano:“CCl_4 处理的大鼠肝脏中纤维蛋白的表达水平”
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Hisamitsu Ohmori,Tadamasa Murakami,Aki Furutani,Ken Higashi,Hideyasu Hirano,Sadao Gotoh et al: "Simultaneous Activation of Heat Shock Protein(hsp70)and Nucleolin Genes during in Vivo and in Vitro Prereplicative stages of Rat Hepatocytes" Exptl.Cell Res.18
Hisamitsu Ohmori、Tadamasa Murakami、Aki Furutani、Ken Higashi、Hideyasu Hirano、Sadao Gotoh 等人:“大鼠肝细胞体内和体外复制前阶段热休克蛋白 (hsp70) 和核仁素基因的同时激活”Exptl.Cell Res。
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Keiroh Utsumi,MotoyuKi Sawada,Seiji Narumiya,Jun Nagamine et al,Hideyasu Hirano,Hiromi Fujiwara: "Adhesion of immature thymocytes to thymic stromal cells through fibronectin molecules and its significance for the induction of thymocyte differentiation" Pr
Keiroh Utsumi、MotoyuKi Sawada、Seiji Narumiya、Jun Nagamine 等人、Hideyasu Hirano、Hiromi Fujiwara:“未成熟胸腺细胞通过纤连蛋白分子粘附到胸腺基质细胞及其对诱导胸腺细胞分化的意义”Pr
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前往

HIRANO Hideyasu的其他基金

Morphlogical and Histological Control in Fibrosis
纤维化的形态学和组织学控制
  • 批准号:
    04807021
    04807021
  • 财政年份:
    1992
  • 资助金额:
    $ 1.09万
    $ 1.09万
  • 项目类别:
    Grant-in-Aid for General Scientific Research (C)
    Grant-in-Aid for General Scientific Research (C)

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