Roles of cellular signal transduction in the defensive mechanism of rabbit stomach

细胞信号转导在兔胃防御机制中的作用

• 批准号: 02670295
• 负责人: OTA Shinichi
• 金额: $ 1.54万
• 依托单位: Tokyo University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1990
• 资助国家: 日本
• 起止时间: 1990 至 1992
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-02670295/
• 关键词: Rabbit gastric mucous cells; Primary culture; mucus secretion; Ca^<2+>; CAMP; ATP; prostaglandin; Defense mechanism; 細胞内Ca^<2+>; cAMP; 培養家兎胃粘液細胞; プロスタクランジンE_2; デオキシコ-ル酸; G蛋白質; sodium fluoride; 胃粘膜; 培養細胞; 粘液分泌; カルシウム; 胆汁酸

We investigated the roles of cellular signal transduction system in the defensive mechanism of rabbit somach using primary culture gastric mucous cells. Rabbit gastric epithelial cells were isolated by enzyme digestion and cultured in F-12 medium with 10% FBS. Cultured cells were mainly composed of mucous cells, as assessed by electronmicroscopy and PAS staining. These cells produced prostaglandin (PG)E_2. Influx of extracellular Ca^<2+> and activation of protein kinase C induced stimulation of PGE_2 release, while modulation of cAMP had no effect. Acid secretagogues such as histamine, carhachol, or gastrin did not alter PGE_2 release, while deoxycholic acid markedly increased PGE_2 release mainly through the influx of extracellular Ca^<2+>. Mucus secretion was also stimulated by the increase of intracellular Ca^<2+>, but not by cAMP. ATP, purinergic P2 receptor agonist increased cellular Ca^- and stimulated mucus secretion and PGE_2 release.In conclusion, intracellular Ca^<2+> might be an important factor in the activation of rabbit gastric defensive mechanism.

我们利用原代培养的胃粘膜细胞研究了细胞信号转导系统在兔胃防御机制中的作用。通过酶消化分离兔胃上皮细胞并在含10%FBS的F-12培养基中培养。通过电子显微镜和PAS染色评估培养的细胞主要由粘液细胞组成。这些细胞产生前列腺素 (PG)E_2。细胞外Ca 2+ 的流入和蛋白激酶C的激活诱导PGE_2释放的刺激，而cAMP的调节没有效果。促酸分泌剂例如组胺、卡哈胆或胃泌素不改变PGE_2释放，而脱氧胆酸主要通过细胞外Ca 2+ 的流入显着增加PGE_2释放。细胞内Ca 2+ 的增加也刺激粘液分泌，但cAMP不刺激粘液分泌。嘌呤能P2受体激动剂ATP增加细胞Ca^-并刺激粘液分泌和PGE_2释放。综上所述，细胞内Ca^2+可能是激活兔胃防御机制的重要因素。

项目成果

SHINICHI OTA,AKIRA TERANO et.al.: "The effects of acid secretagogues on protective agents of gostric cells from adult rabbits in vitro" American Journal of Clinical Gastroenterology.

SHINICHI OTA, AKIRA TERANO 等人：“促酸分泌剂对体外成年兔胃细胞保护剂的影响”美国临床胃肠病学杂志。

Shinichi OTA,et al: "Protective effect of tauroursodeoxycholate against chenodeoxychdate-induced damage" Digestive Disenses & Sciences. 36. 409-416 (1991)

Shinichi OTA 等人：“牛磺熊去氧胆酸盐对鹅去氧胆酸盐引起的损伤的保护作用”消化系统疾病

Shinichi ota,et al: "The effects of acid secretagogues on protective agents of gastric cells from adult rabbits in vitro" American Jounal of Clinical Gastroenterology.

Shinichi ota 等人：“促酸分泌剂对体外成年兔胃细胞保护剂的影响”美国临床胃肠病学杂志。

Shinichi Ota,et al: "Roles of Ca^<2+> and protein kinose C in the regulation of prostaglandn E_2 release by cultured robbit gastric clls" Dig,Dig, & Sci.

Shinichi Ota 等人：“Ca ^ 2 和蛋白激酶 C 在调节培养的兔子胃细胞前列腺素 E_2 释放中的作用”Dig，Dig，