Qualitative change of GTP-binding protein.

GTP结合蛋白的质变。

• 批准号: 02670084
• 负责人: WATANABE Yasuhiro
• 金额: $ 1.41万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1990
• 资助国家: 日本
• 起止时间: 1990 至 1991
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-02670084/
• 关键词: GTP-binding Protein; lithium; Protein kinase; phosphorylation; Qualitative Change; ADP-ribosylation; Pertussis Toxin; リチウム; GTP結合給白

In order to know the qualitative changes of inhibitory GTPbinding(G)protein in vitro, we did ADP-ribosylation of Gi protein by pertussis toxin(islet-activating protein, IAP), an experiment about dissociation of the trimer of the three alpha beta gamma-subunits into alpha -subunits and beta gamma -subunits and western blot using an antibody for Gi-protein. The addition with lithium ion to the protein caused a decrease of the amounts of ADPribosylation of Gi protein by IAP in human platelet membranes and weekened the inhibition'of adenylate cyclase activity by activated Gi-protein, though the dissociation of the three subunits did not change. Also, chronic treatment of manic depressive patients with lithium carbonate decreased the amounts of ADPribosylation by IAP in their platelet membranes. Next the effect of phosphorylation of partially purified Gi-protein by cyclic AMP-dependent protein kinase was studied. The treatment caused a decrease of the amounts of ADP-ribosylation of Gi protein by IAP in human platelet membranes, weekened the phospholipase C activity by activated Gi-protein in differentiated HL-60 cells and inhibited the dissociation of the three subunits induced by Mg2+ and GTPgamma S. Western blot showed no remarkable quantitative changes in Gi protein in the experimental conditions mentioned above. The results strongly suggested that the qualitative changes of Gi-proteins are physiologically and pathologically functional in the regulation of transmembrane signal transduction.

为了了解抑制性GTPBIND（G）蛋白在体外的质量变化，我们通过ttussis毒素（胰岛激活蛋白，IAP）对GI蛋白进行了ADP-核糖基化，这是一个关于使用三个Alpha beta beta beta beta beta goomsuub和alpha-subsub和alpha-subsub和alpha-subsub andpha-subsub andpha-subsub and andpha-subsub和alpha-subs的实验GI-蛋白的抗体。锂离子添加到蛋白质中添加导致IAP在人血小板膜中通过激活的Gi-protein抑制腺苷酸环化酶活性的gi蛋白量减少，尽管这三个亚基的解离并没有改变。同样，碳酸锂躁狂抑郁症患者的长期治疗减少了IAP在其血小板膜中的adpribosylation量。接下来，研究了通过环状AMP依赖性蛋白激酶对部分纯化的Gi蛋白磷酸化的影响。该治疗导致IAP在人血小板膜中通过激活的HL-60细胞中激活的Gi-protein通过激活的Gi-protein抑制了MG2+和GTPGAMMA S.的三种亚基的分离，在分化的HL-60细胞中激活的Gi-s. S. s. s. s. s. s. s. s. s. s. s. s. s. s。结果强烈表明，GI-蛋白的定性变化在生理和病理上在跨膜信号转导的调节中起作用。

项目成果

Y.Watanabe: "Effects of lithium ion on the inhibitory GTPーbinding protein and its coupling response." Cellular Signalling.

Y. Watanabe：“锂离子对抑制性 GTP 结合蛋白及其偶联反应的影响。”

Y. Watanabe: "GTP-binding protein, protein kinase." Hirokawa Shoten, Lecture of Neuroscience.

Y. Watanabe：“GTP 结合蛋白，蛋白激酶。”

Y.Watanabe: "Effects of lithium ion on the inhibitory GTPーbinding protein and its coupling response." Cellular Signalling. 3. 59-64 (1990)

Y. Watanabe：“锂离子对抑制性 GTP 结合蛋白及其偶联反应的影响。” 3. 59-64 (1990)

H.Kawamoto: "Effects of lithium ion on ADP-ribosylation of inhibitory GTP-binding protein by pertussis toxin, islet-activating protein." Eur.J.Pharmacol.Mole.Pharmacol.Sec.206. 33-37 (1990)

H.Kawamoto：“锂离子对百日咳毒素、胰岛激活蛋白抑制性 GTP 结合蛋白 ADP 核糖基化的影响。”

Y.Watanabe: "Effects of lithium ion on the inhibitory GTP-binding protein and its coupling response." Cellular Signalling. 3. 59-64 (1990)

Y.Watanabe：“锂离子对抑制性 GTP 结合蛋白及其偶联反应的影响。”