Surface modification for realizing implantable biosensors

用于实现植入式生物传感器的表面修饰

基本信息

批准号：
02650294
负责人：
IKEDA Kenji
金额：
$ 1.41万
依托单位：
University of Tokyo, Faculty of Medicine
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1990
资助国家：
日本
起止时间：
1990 至 1991
项目状态：
已结题

项目摘要

It is important for implantable immune-sensors by using ion-sensitive field-effect transistor(ISFET)to immobilize antibody-protein with high density and high activity. In this research, we use plasmapolymerized membrane for the career of inunobilized antibody. We use Hexamethyldisiloxane(HMDS), which is excellent in adhesion to silicon substrate, for the materials of organic membrane, and fabricate the membrane by plasma-polymerized method. We introduce SH-groups on the surface of organic membrane as functional group to inunobilize antibody by applying glow-discharge treatment to ethanedithiol having SH-groups. We confirm that hydrohibic surface becomes hydrophobic by this surface modification. The contact angle of the surface tension, which was 125ﾟ with water, turns 52ﾟ after modification. The fact that XPS signals of sulfur is observed only in the modificated membrane, is the evidence that SH-groups are introduced. Cysteine is covalently immobilized by SS-bond on the surface. The an … More tibody(anti-Human Serum Albrffin IgG)is covalently immobilized with crosslinking NH_2-groups of cysteine and SH-groups of antibody F_<ab>' hinge together with bifunctional reagent. The activity immobilized activated antibody density is of the order of 10^<11>[cm^<-2>](the closest density is 2X10^<12>)by enzymeimmunoassey(EIA), which shows high density immobilization. To investigate quantity of non-specific adsorption, organic membrane having no immobilized antibody is similarly measured by EIA. As a result, the membrane introduced SH-groups and Cysteine has less non-specific adsorption than that of NH_2-groups. We measure the changing of surface potential of this immobilized antibody caused by antigen-antibody binding. A surface potential change of the order of 0.1 mV is observed at the antigen concentration of 0.01 - 1 mg/ml. We attain the same result in the case that the antigen coexists with other substances(Bovine Serum). This result suggests the possibility of realizing immune-sensors. Less

通过使用对离子敏感的场效应变压器（ISFET）将具有高密度和高活性的抗体蛋白固定的植入抗性免疫传感器非常重要。在这项研究中，我们使用静脉合膜膜来进行非自然化抗体的职业。我们使用有机膜的材料，使用甲基二硅氧烷（HMD）（HMDS），它在粘合剂上非常适合硅底物，并通过血浆聚合方法制造膜。我们在有机膜表面引入SH组作为功能组，以通过将发光 - 分离处理施加到具有SH组的乙酰硫代醇，从而使抗体不固定。我们证实，通过这种表面修饰，氢苄型表面变为疏水。修饰后，表面张力的接触角（用水为125°）变成52 ﾟ。仅在修饰的膜中观察到硫的XPS信号的事实是引入了SH组的证据。半胱氨酸被SS键共价固定在表面上。 An…更多的胫骨（抗人血清Albrffin IgG）与半胱氨酸的NH_2组共价固定，抗体F_ <ab>'hinge hinge和双功能试剂均铰接。固定活化抗体密度的活性为10^<11> [CM^<-2>]（最接近的密度为2x10^<12>），酶的酶（EIA）显示出高密度固定化。为了研究非特异性吸附的数量，EIA类似地测量了无固定抗体的有机膜。结果，膜引入的SH组和半胱氨酸的吸附较少，而不是NH_2组的吸附。我们测量由抗原抗体结合引起的这种固定抗体的表面电势的变化。在0.01-1 mg/mL的抗原浓度下观察到0.1 mV的表面电势变化。在抗原与其他物质（牛血清）共存的情况下，我们达到了相同的结果。该结果表明有可能实现免疫传感器。较少的