Research of Ovum Capture Inhibitor (OCI) in Endometriosis ; a Study of Anti-OCI Production, Exploitation of Immunological OCI Measurement, Localization and Production of OCI.

卵子捕获抑制剂（OCI）治疗子宫内膜异位症的研究；

基本信息

批准号：
02807152
负责人：
NAKAHASHI Norifumi
金额：
$ 1.28万
依托单位：
Ehime University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1990
资助国家：
日本
起止时间：
1990 至 1991
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-02807152/
关键词：
Endometriosis OCI Lectin 卵管采卵捕獲抑制因子(OCI)

项目摘要

We have found out the OCI (ovum capture inhibitor) abundant in endometriosis peritoneal fluid (PF). OCI makes membranes (OCIRM : OCI related membranes) at Golden hamster fimbria which inhibit oocyte-cumulus pick up in vitro. OCIRM constitutes of fibrous and debris like part. We designed these studies to find out the characterization of OCI.1) To evaluate the OCI molecular weight gel filtration of PF (Sephacryl S-300) was studied. OCI activity was found at 200- 600K Daltons filtrates gatherings.2) Immuno-stain of OCIRM by anti-fibrinogen and anti-PF (immunized rabbit serum by PF<PF was filtrated over 1OOK Daltons with mi11ipore fi1ter>), and anti -OCI activity with both agents were studied. Fibrous part was stained by both but not debtis like part. OCI activity was not suppressed by both agent, but debtis like part was only made.3) With 3 Lectins (Con-Al WGA, SBA) immuno-stainings were studied. OCIRM were stained by Con-A and WGA.4) OCI activity of PF versus total protein and fibrinogen concentration, sediment amount of PF (PF with lectin makes sediments) with lectins (Con-A, WGA, Lentil) were studied (N=13). No relationships were noted between OCI activity and total protein and fibrinogen concentrations, sediment amount with lectins other than Lentil. Conclusions : OCI is constituted of plural large molecules (200- 600K Daltons). Fibrous part of OCIRM was fibrin suspected, but debris like part was lectin binding glycoconjugates suspect. OCI activity appears with fibrinogen and lectin binding substances, but fibrinogen is not necessary and major factor is lectin binding substances.

我们发现OCI（卵子捕获抑制剂）在子宫内膜异位症（PF）中含量丰富。 OCI在Golden Hamster Fimbria上制造膜（OCIR：OCI相关的膜），可在体外抑制卵母细胞 - 肿瘤。 OCIRM构成纤维和碎屑的一部分。我们设计了这些研究以找出OCI.1）的表征，以评估PF的OCI分子量凝胶过滤（Sephacryl S-300）。在200-600k daltons滤液中发现了OCI活性。2）抗纤维蛋白原和抗PF的OCIRM免疫染色（PF <PF免疫化兔血清在1次煮熟的Daltons上用MI11IPORE FI1TER>）过滤，并与两种食物进行了抗-oci活性。纤维部分被两者都像部分一样被染色。 OCI活动并未被两种代理人抑制，但仅造成了债务。3）研究了3种凝集素（Con-Al WGA，SBA）免疫染色。通过CON-A和WGA进行染色。4）PF与总蛋白质和纤维蛋白原浓度的OCI活性，研究了与凝集素（Con-A，WGA，Lentil）的PF的沉积物量（PF凝集素会产生沉积物）（n = 13）。 OCI活性与总蛋白质与纤维蛋白原浓度之间没有任何关系，而除扁豆以外的其他凝集素的沉积物量。结论：OCI由复数大分子（200- 600k daltons）组成。纤维纤维的纤维部分怀疑是纤维蛋白的，但是像凝集素结合糖缀合物的碎屑可疑。 OCI活性与纤维蛋白原和凝集素结合物质一起出现，但纤维蛋白原不是必需的，主要因素是凝集素结合物质。