A Preliminary Physiological Study of Neuronal Networks Underlying the Fish swimming.

鱼类游泳神经元网络的初步生理学研究。

基本信息

批准号：
02806037
负责人：
UEMATSU K.
金额：
$ 1.15万
依托单位：
Hiroshima University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1990
资助国家：
日本
起止时间：
1990 至 1991
项目状态：
已结题

项目摘要

Induction of swimming in restrained fish. We tested two ways to induce a swimming in the carp Cyprinus carpio whenever we wanted. The fish started to swim by a microstimulation to the most caudal region of the midbrain tegmentum with currents less than 5 mu A. The region was small and located just beneath the aquaeductus mesencephali. The induced swimming appeared quite natural and the rhythm changed according to the stimulation current and frequency. An alternative method to induce swimming was sensory stimulation. When the fish was anesthetized with a nembutal injection, a mechanical stimulation to the caudal fin or the peduncle made them swim for several 10 seconds. The dose of nembutal to keep the fish in the state for 2 h was 0.2 mg/100gBW.Fictive Swimming. The activities of the motoneurons during fictive swimming could be monitored from the ventral roots with a suction electrode in the fish that were immobilized with curare and stimulated by one of the above methods.Retrograde La … More belling of Spinal Motoneurons. They were labelled retrogradely with an application of horseradish peroxidase (HRP) onto cut ends of a branch of a ventral root innervating the red muscles or the white muscles. The neurons innervating the red muscles were distributed in the ventral born of the spinal cord, and the mode of their major diameters were 20-40mum. The white muscles were innervated by small motoneurons (20-40mum) and larger ones (60-80mum), that were distributed in the medial region of the ventral horn.Immunocytochemistry of GABA and Glycine. Paraffin sections of the carp spinal cord were stained by using specific antibodies to GABA (gamma-aminobutyric acid) and glycine. Both the antisera stained small cells in the dorsal born and variable-sized cells in the ventral horn. In addition to them, GABA antiserum stained a class of liquor-contacting ependymal cells lining the central canal. Most of the ventral cells including motoneurons were attached with glycineimmunoreactive synaptic boutons. While GABA-immunoreactive synaptic boutons were scarce especially on putative motoneurons. Less

我们测试了两种方法，通过对中脑被盖最尾部区域的电流小于 5 μA 的微刺激来诱导鲤鱼游泳。区域很小，位于中脑导水管下方，诱导游泳显得非常自然，并且根据刺激电流和频率而改变诱导游泳的另一种方法是感觉刺激。鱼用戊巴比妥注射麻醉，对尾鳍或花梗进行机械刺激，使其游动10秒。使鱼保持该状态2小时的戊巴比妥剂量为0.2mg/100gBW。虚构游泳。在虚拟游泳过程中，可以使用用箭毒固定的鱼的吸力电极从腹根监测运动神经元的活动通过使用辣根过氧化物酶（HRP）对支配红色肌肉或白色肌肉的腹侧根分支的末端进行逆行标记。支配红肌的神经元分布于脊髓腹侧，其长径众数为20～40μm；白肌则受小神经元支配。运动神经元 (20-40mum) 和较大的运动神经元 (60-80mum)，分布在腹角的内侧区域。使用 GABA (gamma) 特异性抗体对鲤鱼脊髓的 GABA 和甘氨酸切片进行免疫细胞化学染色。 -氨基丁酸）和甘氨酸。两种抗血清均对背生的小细胞和腹角的不同大小的细胞进行染色。除此之外，GABA 抗血清对中央管内的一类与液体接触的室管膜细胞进行染色，包括运动神经元在内的大多数腹侧细胞都附着有甘氨酸免疫反应性突触纽扣，而 GABA 免疫反应性突触纽扣很少，尤其是在假定的运动神经元上。