Establishment of X-Ray Structure Analysis System for Protein Crystals by Multiwavelength Anomalous Diffraction Methods.

多波长反常衍射方法建立蛋白质晶体X射线结构分析系统。

• 批准号: 02402051
• 负责人: SATOW Yoshinori
• 金额: $ 15.68万
• 依托单位: University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (A)
• 财政年份: 1990
• 资助国家: 日本
• 起止时间: 1990 至 1991
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-02402051/
• 关键词: Protein Crystallography; Multiwavelength Anomalous; X-Ray Crystallography; Diffraction Intensity; Immunoglobulin; Fv Fragment; Synchrotron Radiation; Anomalous Scattering; アミラ-セ阻害剤; ダンシルリジン

The objective of this. research project is to establish a system for direct and rapid structure determination of protein crystals by use of tunable and intense synchrotron X-radiation.First, a dead time correction method for scintillation detectors and automatic optimization procedures for alignment of the X-ray optical components were newly developed for the diffraction intensity measurement system, which consists of the double-crystal monochromator, focussing mirror, and horizontal-type four-circle diffractometer, installed at Photon Factory, National Laboratory for High Energy Physics. The measurement system improved so as to accommodate these procedures is applicable to four-circle diffractometry as well as to oscillation methods, and provides excellent-quality diffraction data at any practical wavelengths. The system has been in open use to various synchrotron experiments. Next, a kappa-axis diffractometer-with a sealed X-ray tube was equipped with a portable crystal-cooling unit … More which decreases radiation damages on protein crystals. Finally, computer programs for processing diffraction data measured at several wavelengths as well as for direct phasing of crystal structure factors were developed. The programs combined with structure refinement, graphics, and other programs were built into a complete system for multiwavelength anomalous diffraction analyses.Crystal Structure of an Fv fragment from mouse anti-dansyllysine immunoglobulin IgG2a has been determined at 2.5 * resolution. The structure clearly elucidates its complementarity determining regions. Structure refinements of the native crystal and a dansyllysine complex crystal are in progress at hislier resolution. Furthermore, crystal structures of ferous spincrossover complexes as well as those of aspartaine IA and IB forms liave been solved in the course of performance tests of the measurement and proprain systems.The systems established in this research project are widely applicable to crystal structure studies of proteins and others. Less

此目的。首先，用于测量系统的X射线光学组件的NT是一种新开发的闪烁时间和自动化方法镜子和水平型四圆衍射仪，在光子厂进行了高能量的实验室。该系统已对各种同步实验进行开放，并带有一个密封的X射线管，带有晶体的便携式晶体冷却单元。开发了晶体结构因子的直接相结合它的互补性确定了天然晶体的结构改进和dansylysine晶体在较大的分辨率下进行的。其他

项目成果

Yoshinori Satow: "Crystal Structures give Information about Activities, Functions, and Evolutions of Proteins." Chemistry Today (Gendaikagaku). 245. 24-31 (1991)

Yoshinori Satow：“晶体结构提供有关蛋白质的活性、功能和进化的信息。”

Janet L. Smith, E. J. Zaulec, J. -P. Wery, & Y. Satow: "Multiwavelength Anomalous Diffraction Analysis of a Large Protein." Proc. of Isomorphous Replacement and Anomalous Scattering.

珍妮特·史密斯 (Janet L. Smith)、E. J. Zaulec、J. -P。

Yoshinori Satow: "Use of Synchrotron Radiation - Characteristics of Synchrotron Radiation and Macromolecular Crystallography." Fundamentals in Crystal Structure Analysis by Diffraction Methods, Japanese Crystallographic Society. 111-127 (1991)

Yoshinori Satow：“同步辐射的使用 - 同步辐射的特征和高分子晶体学。”

Yoshinori Satow(佐藤 能雅): "Optimized Anomalous Dispersion Studies." Acta Crystallographica. A46. C-15 (1990)

Yoshinori Satow：“优化的反常色散研究”。Acta Crystallographa A46（1990）。

Masayoshi Nakasako, Y. Satow et al.: "Crystal Structure of Mouse Anti-Dansyllysine Immunoglobulin Fv Fragment"

Masayoshi Nakasako、Y. Satow 等人：“小鼠抗丹磺酰赖氨酸免疫球蛋白 Fv 片段的晶体结构”