Cooperative Research in the Field of Plant Bioscience Between Japan and Foreign Countries, Especially European Countries and USA

日本与国外特别是欧美国家在植物生物科学领域的合作研究

基本信息

批准号：
02044022
负责人：
HARADA Hiroshi
金额：
$ 1.92万
依托单位：
University of Tsukuba
依托单位国家：
日本
项目类别：
Grant-in-Aid for international Scientific Research
财政年份：
1990
资助国家：
日本
起止时间：
1990 至无数据
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-02044022/
关键词：
Transformation Gene Expression Secondary Metabolism Glycoproteins Higher Plants International Cooperative Research

项目摘要

Cooperative research between Japan and foreign countries, especially U.S.A. and European countries, was carried out in plant bioscience field.1. On transformation : Several transformation methods for higher plants have already been developed and some successful results have been reported in model plants. However, only limited number of reports are available on transformation of agriculturally important plants. Therefore, cooperative research was carried out on the transformation of potato and sweet potato. Our results showed that Ri plasmid was an adequate vector for introducing foreign genes such as kanamycine resistant and GUS genes. Studies on transformation of Streptocarpus, an important material for investigating flower bud differentiation, has been continued.2. On glycoproteins : A carrot secretory glycoprotein GP57 isolated by Tsukuba group was compared with a similar glycoprotein isolated in Wageningen Agricultural University. The expression pattern of the both glycoproteins is comparable, but antiserum for GP57 did not react to the another. The gene expression of GP57 was examined in FMI of Switzerland. The result showed that GP57 gene expression was wound-inducible and thought to have a physiological function in defense mechanism.3. On control mechanism of gene expression : Expression of agropine synthase gene which was identified on T-DNA of Ti plasmid was controlled by the promoter region of the gene and showed a specificity related to cell division. Homology search on nucleotide sequence among various T-DNA genes was carried out in Leiden University and showed no homology among them.4. On secondary metabolites : Production of some secondary metabolites is related to tissue and organ differentiation. Tropane alkaloid production was directly related to root differentiation. Detailed examination on synthetic pathway of secondary metabolites was carried out in cooperation and some new results were obtained.

日本和国外，尤其是美国和欧洲国家之间的合作研究是在植物生物科学领域进行的。1。关于转型：已经开发了几种高等植物的转化方法，并且在模型植物中已经报道了一些成功的结果。但是，仅有关农业重要植物转型的报告数量有限。因此，对马铃薯和地瓜的转化进行了合作研究。我们的结果表明，RI质粒是引入外源基因（例如kanamycine抗性基因和GUS基因）的足够载体。链球菌的转化研究是研究花蕾分化的重要材料。2。在糖蛋白上：Tsukuba组分离的胡萝卜分泌糖蛋白GP57与在Wageningen农业大学中分离的类似糖蛋白进行了比较。两种糖蛋白的表达模式都是可比的，但是GP57的抗血清与另一种抗血清没有反应。在瑞士FMI中检查了GP57的基因表达。结果表明，GP57基因表达是可诱导的，并被认为在防御机制中具有生理功能。3。关于基因表达的控制机制：在Ti质粒的T-DNA上鉴定出的农业合酶基因的表达受基因的启动子区域控制，并显示出与细胞分裂有关的特异性。在莱顿大学进行了各种T-DNA基因之间核苷酸序列的同源搜索，其中没有显示同源。4。关于次生代谢物：一些次生代谢产物的产生与组织和器官分化有关。 Tropane生物碱的产生与根分化直接相关。在合作中对继发代谢物的合成途径进行了详细检查，并获得了一些新的结果。