The Affects of Oral Environment on Restoratives

口腔环境对修复剂的影响

基本信息

批准号：
01571045
负责人：
SHINTANI Hideaki
金额：
$ 1.41万
依托单位：
Hiroshima University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1989
资助国家：
日本
起止时间：
1989 至 1990
项目状态：
已结题

项目摘要

Seven well-defined proteins known to be contained in saliva, i. e. albumin, lgA, gamma-Globulin, Lysozyme, alpha-Amylase, Collagen, Mucin were covalently coupled to glass slides. Mucin and whole saliva treated with neuraminidase and fractons obtained by Sephadex G-200 gel filtration of whole saliva were also studied. The adherence of Streptococcus sanguis ATCC 10556, S. sanguis ATCC 10557, S. mutans Ingbrift and S. mutans OMZ 176, to these protein coupled glass slides was studied to assess the relative contribution to bacterial adhesion played by electrostatic charge, hydrophobicity and the presence of sialic residues on a surface.The numbers of bacteria adhered to these glass slides were stabstcally compared with zeta-potentials and contact angles of bacteria and protein-coupled glass slides. As a result a clear correlation between numbers of adherent cells and zeta-potentials' of protein-coupled glass slides was observed, suggesting that long range non-specific electrostatic interactions are important for adherence. In addition, it was also suggested that in S. sanguis, specific neuraminidase-sensitve interacton and non-specific hydrophobic interaction play some role in adherence, however, electrical attracton and short-range neuraminidase-sensitive interaction seem to override hydrophobic interaction.Components in whole saliva were fractonated by gel filtration. Three major peaks were obtained and one peak contained Mucins and lgA provided a better interface for adherence of S. sanguis than other peaks that not contained Mucins. And when this peak were treated with neuraminidase, then the adherence of S. sanguis were decreased. These results also indicated that the sialic asid residues of mucin appeared to play an important role in the adherence of S. sanguis.

七种已知已知包含在唾液中的七个明确的蛋白质，i。 e。白蛋白，LGA，γ-球蛋白，溶菌酶，α-淀粉酶，胶原蛋白，粘蛋白共价耦合到载玻片。还研究了用神经氨酸酶和通过Sephadex G-200凝胶过滤获得的整个唾液的粘蛋白和整个唾液。研究了这些蛋白质耦合的玻璃载玻片对这些蛋白质耦合的载玻片的粘附，S。SanguisATCC 10556，S。SanguisATCC 10557，S。Mutans Injbrift和S. mutans Omz 176，以评估对细菌粘附的相对贡献。与细菌和蛋白质耦合载玻片的Zeta势力和接触角相比。结果，观察到了蛋白质偶联载玻片的粘附细胞数量与Zeta-potitys之间的明显相关性，这表明长期非特异性的非特异性静电相互作用对于依从性很重要。 In addition, it was also suggested that in S. sanguis, specific neuraminidase-sensitve interacton and non-specific hydrophobic interaction play some role in adherence, however, electrical attracton and short-range neuraminidase-sensitive interaction seem to override hydrophobic interaction.Components in whole saliva were fractonated by gel filtration.获得了三个主要峰，一个峰含有粘液素，LGA比其他不包含粘蛋白的峰提供了更好的S. sanguis的界面。当用神经氨酸酶处理该峰时，sanguis的依从性降低。这些结果还表明，粘蛋白的唾液ASID残基似乎在S. sanguis的依从性中起重要作用。