Studies of the phagocytic of Reticulo-Endothelial System in rat liver following thermal injury.

热损伤后大鼠肝脏网状内皮系统吞噬细胞的研究。

基本信息

批准号：
01570864
负责人：
OHTANI Minako
金额：
$ 1.41万
依托单位：
Hiroshima University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1989
资助国家：
日本
起止时间：
1989 至 1991
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-01570864/
关键词：
Shock phase in burns Kupffer cells Phagocytic activity Endotoxin White pulp of Spleen Cell necrosis 脾臓白脾髄リンパ濾胞濾胞辺縁帯貪食能遊離ヘモグロビンマクロファ-ジ

项目摘要

The reticuloendothelial system(RES)is an essential component of the non-specific host defense system which clears circulating debris, such as aggregated protein, damaged cells and fibrin from the bloodstream, in addition to removing blood-borne bacteria.We studied changes in plasma haemoglobin levels and morphology of Kupffer cells in rats following lethal thermal injury. The plasma haemoglobin levels rapidly increased to a maximum level 15 minutes after injury and then rapidly decreased with time. However, the values were still higher 5 hours after injury than those found before injury. Soon after burning the Kupffer cells phagocytized not only circulating cell debris including erythrocyte membranes and degenerate leucocytes but also large amounts of haemoglobin. This phagocytized haemoglobin is considered to inhibit the generation of bactericidal-free radicals and to depress Kupffer cell function. The number of Kupffer cells was markedlly decreased 5 hours after thermal injury, and probably relates to the persistent depletion of the reticuloendothilial system function which follows lethal thermal injury. In contrast to the decreased number of Kupffer cells, a small number of monocytic cells appeared in the sinusoidal spaces and adhered to the endothelial cells.Our findings suggest that blood monocutes might migrate to the sinusoidal wall and transform into Kupffer cells. We also studied the effect of endotoxin on Kupffer cells in the rat liver following 30% thermal injury of body surface area. The Kupffer cells were markedly degenerate in the early stage.Structural changes of the white pulp of the spleen were investigated in rats following thermal injury. Tingible bodies and tingible body macrophages were abundant in germinal center, follicular are and perifollicullar zone.

网状内皮系统 (RES) 是非特异性宿主防御系统的重要组成部分，除了清除血液中的细菌外，它还能清除血液中的循环碎片，例如聚集的蛋白质、受损的细胞和纤维蛋白。我们研究了血浆中的变化致死热损伤后大鼠库普弗细胞的血红蛋白水平和形态。血浆血红蛋白水平在受伤后15分钟迅速上升至最高水平，然后随着时间的推移迅速下降。然而，受伤后 5 小时的数值仍然高于受伤前的数值。燃烧后不久，库普弗细胞不仅吞噬循环细胞碎片，包括红细胞膜和退化白细胞，还吞噬大量血红蛋白。这种被吞噬的血红蛋白被认为可以抑制杀菌自由基的产生并抑制库普弗细胞功能。热损伤后5小时库普弗细胞数量显着减少，可能与致死性热损伤后网状内皮系统功能持续耗竭有关。与库普弗细胞数量减少相反，少量单核细胞出现在血窦间隙并粘附于内皮细胞。我们的研究结果表明，血液单核细胞可能迁移到血窦壁并转化为库普弗细胞。我们还研究了体表面积 30% 热损伤后内毒素对大鼠肝脏库普弗细胞的影响。早期库普弗细胞明显退化。研究热损伤后大鼠脾白髓的结构变化。生发中心、毛囊区和毛囊周围区有丰富的可着色体和可着色体巨噬细胞。