Study of Relationships Between Ankylosing Spinal Hyperostosis and Retinoid

强直性脊柱骨质增生与类维生素A关系的研究

基本信息

批准号：
01570834
负责人：
TERAYAMA Kazuo
金额：
$ 1.34万
依托单位：
Shinshu University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1989
资助国家：
日本
起止时间：
1989 至 1990
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-01570834/
关键词：
Retinoid Vitamin A Ankylosing Spinal Hyperostosis Ossification of Posterior Longitudinal Ligament of the Spine レチノイド強直性脊髄骨増殖症後縦靭帯骨化症

项目摘要

The cause and pathogenesis of ankylosing spinal hyperostosis have not been clearly understood. The action of retinoids on the disease was analyzed for the causative mechanisms. The following are the methods and results of this study.1. A retinoid was excessively and continually administered to rats in order to learn whether or not symptoms similar to ankylosing spinal hyperostosis appear. The concentration of retinoids in the blood was determined, and the values were contrasted to the X-ray findings for each rat. Although findings similar to the ossification of ligaments were observed sporadically, the incidence of ossification was independent of the concentration of retinoids, and the amounts and terms of the administration. The bone mineral volume tended to be reduced when the total administrative amounts of retinoids was increased. The hypothesis, that retinoids play a causative role in the onset of hyperostosis, was not supported by these results. Conversely, because a reduction of the bone mineral volume was frequently observed, the participation of retinoids in the metabolism of bones seems obvious.2. The distribution of vitamin A storing cells in the calcaneus tendon in rats was investigated. These cells were not observed in the tendon, but an increase of these cells was observed in the liver as the administration of retinoids increased. It was revealed that in the skeletal ligaments there were few cells for the reservation of and/or to be a target cell of retinoids.3. The influence of retinoids on a cultured bone was investigated. Growth inhibition on the femoral and tibial bones of chicken fetuses was observed to depend on the concentration of retinoids. This fact suggests that retinoids inhibit the action of cartilagenous cells.4. Retinoids in the serum of patients with ankylosing spinal hyperostosis and controls was determined. The relationship between the concentration of retinoids and the disease was not clearly determined.

强直性脊柱骨质增生的病因和发病机制尚未明确。分析了类维生素A对该疾病的作用及其致病机制。本研究的方法和结果如下： 1．为了了解是否出现类似于强直性脊柱骨质增生的症状，对大鼠进行了过量且连续的类维生素A注射。测定血液中类维生素A的浓度，并将该值与每只大鼠的X射线检查结果进行对比。尽管偶尔观察到类似于韧带骨化的结果，但骨化的发生率与类维生素A的浓度、给药量和时间无关。当类维生素A的总给药量增加时，骨矿物质量趋于减少。这些结果并不支持类维生素A在骨质增生的发生中起因果作用的假设。相反，由于经常观察到骨矿物质量的减少，类视黄醇对骨骼代谢的参与似乎很明显。 2．研究了大鼠跟腱中维生素 A 储存细胞的分布。在肌腱中没有观察到这些细胞，但随着类视黄醇给药的增加，在肝脏中观察到这些细胞的增加。结果表明，在骨骼韧带中，保留类维生素A和/或类维生素A的靶细胞的细胞很少。 3．研究了类维生素A对培养骨的影响。观察到对鸡胎儿股骨和胫骨的生长抑制取决于类维生素A的浓度。这一事实表明类维生素A抑制软骨细胞的作用。4．测定了强直性脊柱骨质增生患者和对照者血清中的类维生素A。类维生素A浓度与疾病之间的关系尚未明确确定。