Quantitative Study of Stretch Channel Activation.

伸展通道激活的定量研究。

• 批准号: 01570044
• 负责人: SOKABE Masahiro
• 金额: $ 1.34万
• 依托单位: Nagoya University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1989
• 资助国家: 日本
• 起止时间: 1989 至 1990
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-01570044/
• 关键词: Stretch activated channel; Membrane tension; Membrane capacitance; Patch clamp; Video microscopy; Cytoskeleton; Stretch Activated Channel; ビデオマイクロコピィ; 細胞骨格系; ビデオマイクロスコピ-; 刺激-応答特性

In these years, stretch activated (SA) channels have been found in most cells. Though we know little about the physiological functions of this type of channel, their ubiquitous distribution suggests that they have fundamental roles in cells. To investigate the gating mechanism of SA channels we have to measure membrane tension, which has not been done yet. According to Laplace's law the membrane tension is determined by transmembrane pressure and the curvature of the patch. The aim of this research project was to visualize the patch and to make simultaneous measurements of the patch shape and SA channel activity during pressure change. The obtained results are as follows :1, We could visualize patch membranes with a resolution of 15 um/pixel.2, The curvature of the patch was changeable depending on pipette pressure.3, The patch membranes followed Hook's law extended to two dimension and the elastic constant of typical patches was 50 dyn/cm.4, The SA channel activity was well correlated with tension but not pressure.5, It was found that the patch thickness did not change during tension change suggesting that the tension bearing element was cytoskeleton attached to SA channel proteins, but not lipids.

在这些年中，在大多数细胞中都发现了拉伸激活的（SA）通道。尽管我们对这种类型的通道的生理功能知之甚少，但它们的无处不在分布表明它们在细胞中具有基本作用。为了研究SA通道的门控机制，我们必须测量膜张力，这尚未完成。根据拉普拉斯定律，膜张力取决于跨膜压力和斑块的曲率。该研究项目的目的是可视化斑块，并在压力变化期间同时测量斑块形状和SA通道活动。所获得的结果如下：1，我们可以以15 um/pixel的分辨率可视化斑块膜。2，斑块的曲率根据移液压的压力而变化。3，斑块膜遵循钩子延伸到二维的定律到二维的定律，典型的斑块的弹性常数为50 dyn/cmm.4 dyn/cmm.4，但在plosit.4，sa ins pressions pats not whers partsions pats inters partsions pats intimens where dies not。张力变化表明张力轴承元件是附着在SA通道蛋白上的细胞骨架，而不是脂质。

项目成果

Sachs,F.: "Stretch activated ion channels and membrane mechanics." Neurosci.Res.12. S1-4 (1990)

Sachs,F.：“拉伸激活离子通道和膜力学。”

Sokabe,M.: "Effects of cyclic nucleotides and calcium on transduction and encoding processes in frog muscle spindle" Brain Res.443. 254-260 (1988)

Sokabe,M.：“环核苷酸和钙对青蛙肌梭转导和编码过程的影响”Brain Res.443。

Sokabe,M.: "Towards molecular mechanism of the gating in stretch activated channels.In “Comparative Aspect of Mechanoreceptin"(Ed.F.Ito)," Springer Verlag,

Sokabe, M.：“探讨拉伸激活通道中门控的分子机制。在“Mechanoreceptin 的比较方面”(Ed.F.Ito)，”Springer Verlag，

Sokabe,M.: "The structure and dynamics of patch clamped membranes" J.Cell Biol.

Sokabe,M.：“膜片钳膜的结构和动力学”J.Cell Biol。

Ito, F.: "Effects of intracellular calcium on the frog muscle spindle in relation to cyclic AMP action.In“Mechanoreceptor"" Plenum Press, 195-199 (1988)

Ito, F.：“细胞内钙对青蛙肌梭的影响与环磷酸腺苷作用有关。In“Mechanoreceptor”” Plenum Press，195-199 (1988)