Studies of Neuronal Properties in the Reaggregate Cultures of Rat Retinane

大鼠视黄烷重新聚集培养物中神经元特性的研究

基本信息

批准号：
63570064
负责人：
AKAGAWA Kimio
金额：
$ 1.34万
依托单位：
Okazaki National Research Institutes (1989-1990)Saitama Medical University (1988)
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1988
资助国家：
日本
起止时间：
1988 至 1990
项目状态：
已结题

项目摘要

Retinal reaggregate cultures were established from new born rats. All the experiments were carried out 10-15 days after plating.Histological studies revealed that selective layer formation were reconstituted in the reaggegates. Since these histological properties resembled those in vivo, electro-physiological experiments were carried out in order to study the possible presence of neural net works in vitro, which might be rudiMentary for retinal functions. It was possible to establish cell attach configuration on the cells in the outermost layer. Under a dark condition, about one fifth of the cells in the outermost layer showed spontaneous discharges. Interestingly, a few neurons increased the frequency of discharges when light stimuli was given to the whole reaggregate. The proportion of the cells with such light-response like activity was 1.6%. Since it was already reported by an immunohistological study that most cells in the outermost layer were amacrine cells and the photoreceptors were exclusively localized in the central region, these results indicated that functional connections were reconstituted between the photoreceptors and the amacrine cells in the reaggregate cultures. These results also demonstrated that the reaggregate cultures could provide interesting models to study the development of neural networks in vitro.

从新生的老鼠建立了视网膜重新培养文化。所有实验均在板条后10-15天进行。组织学研究表明，选择性层的形成是在试剂盖中重构的。由于这些组织学特性类似于体内的特性，因此进行了电生理实验，以研究体外神经网的可能存在，这可能是视网膜功能的基本性。可以在最外层的单元格上建立单元格附加构型。在深色条件下，最外层的大约五分之一的细胞显示出自发的放电。有趣的是，当对整个重新核对光刺激时，一些神经元增加了放电的频率。这种光性类似活性的细胞比例为1.6％。由于一项免疫组织学研究已经报道了最外层的大多数细胞是无结合细胞，并且感光体仅位于中央区域中，因此这些结果表明功能连接是在重新培养的培养物中的光感受器和无聚氨酸细胞之间重组的。这些结果还表明，重新培养的培养物可以提供有趣的模型来研究体外神经网络的发展。