Development and differentiation of retinal pigment epithelial cells.

视网膜色素上皮细胞的发育和分化。

基本信息

批准号：
63570006
负责人：
HIROSAWA Kazushige
金额：
$ 1.34万
依托单位：
The University of Tokyo
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1988
资助国家：
日本
起止时间：
1988 至 1990
项目状态：
已结题

项目摘要

Chick retinal pigment epithelial cells were studied by immunohistochemistry with monoclonal antibodies obtained in my laboratory. Results are divided into two parts.1. Development of RPE cells :Photoreceptor cells had no inner and outer segments on the 8th day in ovo. The inner segments have been formed on the 15th day and the outer segments on the 19th day. PRE sheets had short and blunt apical processes on the 8th day, elongated and slender ones on the 15th day, and well-developed and melanin-containing processes on the 19th day. RPE of the 19th day had numerous bundles of actin filaments associated with melanin pigments in the basal portion of the apical processes and in the apical cytoplasm. In rhodamine-phalloidin preparations, intense fluorescence was localized in the RPE apical processes and cytoplasm on the 19th day. We concluded that RPE apical processes develop in accordance with the photoreceptor development.2. Monoclonal bodies specific to the endoplasmic reticulum of RPE :Among the antibodies obtained, two (S5D8 and S5H8) stained the cytoplasm of epithelial cells of the animals examined in finally granular pattern and recognized a 63kD protein in retinal pigment epithelial cells. These antibodies, however, did not react with the rest of the chick eye (cornea, iris, ciliary body, lens and sclera) nor with extraocular tissues (liver, kidney, intestine, brain, testis, adrenal gland, heart, gizzard and skeletal muscle). Immunoelectron microscopy revealed that the antigen was localized in the endoplasmic reticulum of retinal pigment epithelial cells.

通过在我的实验室中获得的单克隆抗体，通过免疫组织化学研究了鸡视网膜色素上皮细胞。结果分为两部分。1。 RPE细胞的发育：感光细胞在OVO的第8天没有内部和外部段。内部段是在第15天和第19天的外部段形成的。预纸在第8天进行了简短而钝的顶端过程，在第15天进行了细长和细长的过程，并且在第19天，富有发达和黑色素的过程。第19天的RPE有许多与黑色素色素相关的肌动蛋白丝，在顶端过程的基础部分和顶端细胞质中。在花道胺 - 甲状腺素制剂中，在第19天，将强烈的荧光定位于RPE顶端过程和细胞质中。我们得出的结论是，RPE顶端过程根据光感受器的开发而发展。2。 RPE内质网的单克隆体：在获得的抗体中，两种（S5D8和S5H8）染色的动物的上皮细胞的细胞染色，最终以颗粒状的模式检查，并识别出视网膜色素上皮细胞中的63kD蛋白。然而，这些抗体与其余的鸡眼（角膜，虹膜，纤毛，晶状体和巩膜）也没有反应，也不反应于外眼组织（肝脏，肾脏，肠，肠，脑，脑，睾丸，肾上腺，心脏，心脏，gizz和骨架）肌肉）。免疫电子显微镜表明，抗原位于视网膜色素上皮细胞的内质网中。