Modern exercise suite for basic research in small rodents

用于小型啮齿动物基础研究的现代运动套件

• 批准号: RTI-2021-00201
• 负责人: Holloway, Graham
• 金额: $ 7.29万
• 依托单位: University of Guelph
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments
• 财政年份: 2020
• 资助国家: 加拿大
• 起止时间: 2020-01-01 至 2021-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=718817
• 关键词: Modern; exercise; suite; basic; research

Our combined research programs focus on elucidating novel mechanisms controlling cellular metabolism in several tissues. Exercise represents a rapid perturbation to metabolic homeostasis, and therefore has become the cornerstone of our mechanistic research pertaining to metabolic control, and thus is absolutely essential for each independent research program. We are requesting funds for a small rodent exercise suite costing ~$100,000 ($73,870 USD). This equipment consists of a treadmill accommodating 6 rats or 12 mice simultaneously to replace broken equipment, as well as a treadmill and running wheels that are modular attachments for our existing Comprehensive Laboratory Animal Monitoring System (CLAMS) metabolic caging system. This modular infrastructure will be used with our existing CLAMS unit and allow us to measure energy expenditure and substrate oxidation during exercise, significantly enhancing the quality of data that is generated. This infrastructure is ideal for our purposes as the running wheels fit inside our existing single animal housing, allowing us to regulate exercise duration and intensity within the mouse enclosure while continuing to monitor whole body fuel utilization. Moreover, the modular treadmills (one for mice and another for rats) interface with the CLAMs unit to determine the aerobic capacity of animals. We require the requested system for two primary reasons: 1) the equipment is urgently needed to maintain our research productivity and HQP training as two of our current rodent treadmills are broken, and because of their advancing age cannot be fixed, and 2) the requested equipment will expand our capabilities that will enable us to study energy expenditure and fuel utilization more accurately/comprehensively. As a result, the requested equipment represents a critical component of advancing our research programs and ensuring HQP have access to the necessary technology to address leading edge questions in our field. In addition, the requested equipment will support the research of ~13 NSERC-funded laboratories in the Department of Human Health and Nutritional Sciences (HHNS) with >50 HQP currently using our existing small rodent metabolic suite. This is an extremely cost-effective proposal, as the requested equipment will optimize our existing equipment, but only represents <5% of the total investment (NSERC+CFI) in our departmental metabolic animal facility in the past 10 years.

我们的联合研究项目侧重于阐明控制多种组织中细胞代谢的新机制。 运动代表着对代谢稳态的快速扰动，因此已成为我们有关代谢控制的机械研究的基石，因此对于每个独立研究项目都是绝对必要的。 我们正在申请资金用于建造一个小型啮齿动物运动套件，成本约为 100,000 美元（73,870 美元）。该设备由一台可同时容纳 6 只大鼠或 12 只小鼠的跑步机组成，用于更换损坏的设备，以及一台跑步机和跑轮，它们是我们现有的综合实验动物监测系统 (CLAMS) 代谢笼养系统的模块化附件。这种模块化基础设施将与我们现有的 CLAMS 装置一起使用，使我们能够测量运动期间的能量消耗和底物氧化，从而显着提高生成的数据的质量。该基础设施非常适合我们的目的，因为跑轮适合我们现有的单一动物房屋内，使我们能够调节小鼠围栏内的运动持续时间和强度，同时继续监测全身燃料利用率。此外，模块化跑步机（一个用于小鼠，另一个用于大鼠）与 CLAM 装置连接以确定动物的有氧能力。 我们需要所请求的系统有两个主要原因：1) 迫切需要该设备来维持我们的研究生产力和 HQP 培训，因为我们现有的两台啮齿动物跑步机已损坏，并且由于其老化无法修复，2) 所请求的系统设备将扩展我们的能力，使我们能够更准确/全面地研究能源支出和燃料利用。因此，所需的设备是推进我们的研究计划并确保总部能够获得必要的技术来解决我们领域的前沿问题的关键组成部分。此外，所需设备将支持人类健康和营养科学部 (HHNS) 约 13 个 NSERC 资助的实验室的研究，其中超过 50 个 HQP 目前正在使用我们现有的小型啮齿动物代谢套件。 这是一项极具成本效益的提案，因为所需的设备将优化我们现有的设备，但仅占我们部门过去 10 年代谢动物设施总投资 (NSERC+CFI) 的 <5%。