Development of the Mehtod for Separating and Determining a Small Amount of Protein by the Use of Chemiluminescence Reaction.

利用化学发光反应分离和测定少量蛋白质的方法的开发。

• 批准号: 63550567
• 负责人: HARA Tadashi
• 金额: $ 1.54万
• 依托单位: Doshisha University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1988
• 资助国家: 日本
• 起止时间: 1988 至 1990
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-63550567/
• 关键词: Protein; Separation; Determination; Chemiluminescence; Detector; Immunosensor; Flow-injection analysis; Ion-exchange chromatography; キャピラリ-電気泳動; 化学発光検出器; 分離分析; 検出; トリプトファン; 5ーヒドロキシインド-ル酢酸; 標識; 化学発光反応; 分離・定量法; 液体クロマトグラフィ-; フローインジェクション分析 /

The following results were obtained during 1988 - 1990,1. Development of chemiluminescent detectors :(1) The flow-injection analysis of protein using ultrasonic chemiluminescence of luminol.Bull. Chem. Soc. Jpn., 61, 2996(1988).(2) Improvement in FIA system for determining small amounts of proteins with chemiluminescence detection. BUNSEKI KAGAKU, 38, T100(1989).2. Connection of a separation column for proteins with a chemiluminescence detector :(1) High sensitive matal chelate affinity chromatography.Bull. Chem. Soc. Jpn., 61, 2779(1988).(2) A zeolite column-chemiluminescence detection system for the separation and the determination of a protein mixture.Bull. Chem. Soc. Jpn., 62, 1501(1989).(3) High-sensitive detection of proteins by an ion-exchange chromatograph-chemiluminescence detector system.Bull. Chem. Soc. Jpn., 63, 770(1990).3. Development of an immunosensor using chemiluminescence reaction :(1) A highly sensitive fiber-optic immunosensor using a metal-complex compound as a chemiluminescent catalyst.Bull. Chem. Soc. Jpn., 62, 2844(1989).4. Miscellaneous :(1) Experimental consideration on the chemiluminescence detection of protein using the 1,10-phenanthroline-hydrogen peroxide-copper (II) system.Bull. Chem. Soc. Jpn., 63, 2423(1990).(2) Chemiluminescence of L-tryptophan in copper (II) - hydrogen peroxide - potassium sodium tartrate system.Chem. Lett., 843(1990).(3) Chemiluminescence analyses of biological constituents using metal-complex catalysts. A review.Anal. Sci., 6, 797(1990).(4) Additional two papers in THE SCI. AND ENG. REVIEW OF DOSHISHA UNIV.

1988 年至 1990 年期间获得了以下结果1。化学发光检测器的研制：(1)利用鲁米诺超声化学发光进行蛋白质的流动注射分析。Bull。化学。苏克。 Jpn., 61, 2996(1988).(2) 改进FIA系统，用于通过化学发光检测测定少量蛋白质。文石科学，38，T100(1989).2。蛋白质分离柱与化学发光检测器的连接：(1)高灵敏度金属螯合物亲和层析。Bull。化学。苏克。 Jpn., 61, 2779(1988).(2) 用于分离和测定蛋白质混合物的沸石柱化学发光检测系统。Bull。化学。苏克。 Jpn., 62, 1501(1989).(3) 通过离子交换色谱-化学发光检测器系统对蛋白质进行高灵敏度检测。Bull。化学。苏克。日本，63, 770(1990).3。利用化学发光反应的免疫传感器的开发：(1)利用金属络合物作为化学发光催化剂的高灵敏度光纤免疫传感器。Bull。化学。苏克。日本，62，2844（1989）.4。其他：（1）使用1,10-菲咯啉-过氧化氢-铜（II）系统化学发光检测蛋白质的实验考虑。Bull。化学。苏克。 Jpn., 63, 2423(1990).(2)铜(II)-过氧化氢-酒石酸钾钠体系中L-色氨酸的化学发光。 Lett., 843(1990).(3) 使用金属络合物催化剂对生物成分进行化学发光分析。评论。肛门。 Sci., 6, 797(1990).(4) SCI 中另外两篇论文。和工程。同志社大学的评论

项目成果

Tadashi Hara et al.: "A Highly Sensitive FiberーOptic Immunosensor Using a MetalーComplex Compound as a Chemiluminescent Catqlyst." Bull.Chem.Soc.Jpn.62. 2844-2848 (1989)

Tadashi Hara 等人：“使用金属络合物作为化学发光催化剂的高灵敏度光纤免疫传感器”Bull.Chem.Soc.Jpn.62 (1989)。

Tadashi Hara et al.: "The Determination of a Small Amount of a Biological Constituent by the Use of Chemiluminescence.XVII.Experimental Consideration on the Chemiluminescence Detection of Protein Using the 1,10ーPhenanーthrolineーHydrogen PeroxideーCopper(II)

Tadashi Hara 等人：“使用化学发光测定少量生物成分。XVII.使用 1,10-菲-咯啉-过氧化氢-铜（II）进行蛋白质化学发光检测的实验考虑”

塚越一彦: "化学発光反応を用いる微量タンパク質のフロ-インジェクション分析法の改良" 分析化学. 38. T100-T103 (1989)

Kazuhiko Tsukagoshi：“利用化学发光反应改进微量蛋白质的流动注射分析方法”分析化学 38。T100-T103 (1989)

Tadashi Hara et al.: "Investigation of the Complex Formation Between Cobalt(III) and Bovine Serum Albumin by Use of Chemiluminescence Detection." THE SCI.AND ENG.REVIEW OF DOSHISHA UNIV.29. 1-6 (1988)

Tadashi Hara 等人：“利用化学发光检测研究钴 (III) 和牛血清白蛋白之间的复合物形成。”

Tadashi Hara.: Bull.Chem.Soc.Jpn.61. 2996-2998 (1988)

原正：Bull.Chem.Soc.Jpn.61。