Search of molecules involved in the biological clock system and the primordial visual system.

寻找参与生物钟系统和原始视觉系统的分子。

• 批准号: 01304060
• 负责人: HASEGAWA Kenji
• 金额: $ 11.71万
• 依托单位: School of Medicine, Kitasato University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Co-operative Research (A)
• 财政年份: 1989
• 资助国家: 日本
• 起止时间: 1989 至 1991
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-01304060/
• 关键词: Biological Clock; Primordial visual system; Paramecium; Pineal gland; Suprachiasmaticnuclei; Protein Kinasc C; Somatostafin; vasoactive peptyde; 視交叉上核; ショウジョウバエ; メラトニン; cGMP; ロトプシン; 11cis型レチナ-ル; バソプレシン; 概日リズム; 光受容系; エネルギ-伝達系; 光情報伝達系; ロド

1. In Paramecium :1) there was found a rhodopsin-like protein binding retinal, which may serve as a photoreceptive pigment of the specimen,2) concentrations of cAMP and cGMP were high during the day and low at night, characteristics of which sustained under the condition of constant darkness (DD), and3) fluctuation of protein kinase C appeared to be basically the same as those of cGMP and cAMP.2. In Drosophilla :1) two mutants, "Toki" and "Ritsu" were newly isolated, the circadian periods of which were different from the wild type Drosophilla. Genetic and chronobiological characteristics of the mutants were analyzed. In addition, one mutational line which did not show convincing circadian periodicity was isolated using the enhancer trap method.2) compound eyes appeared not to function as the photo-transduction system for the biological clock system of the organism.3. Other animals :1) Even a small piece of the Newt pineal gland left, can serve as synchronizer of the circadian rhythm, b … More ut not as generator of the rhythm.2) The melatonin in the eyes of Quail shows a convincing rhythm in an light/dark cycle, which possibly be involved in the rhythmic cell division of retinal pigment epithelial cell, and3) The C3H mouse genetically lacking rod cell showed the inhibition rate of the melatonin synthesis about 100 time less than that of the normal CBA mouse, when stimulated by the white light, the difference of which could be attributed to the presence of rhodopsin in the CBA mouse.4. The amounts of peptides in the suprachiasmatic nuclei, which are known to be the centers of mammalian circadian clock, were measured by the enzyme-linked immunodetection. Following facts were clarified :1) Upon stimulation by light, the vasoactive intestinal peptide (VIP) decreased and the gastrin-releasing peptide increase, while both peptides dld not show circadian rhythm in DD.2) The vasopressin (AVP) and Somatostatins (SS) exhibited convincing circadian in DD. The amount of mRNA of SS rose to the maximum at ct 0 and fell to the minimum at 12. Less

1。在映射中：1）发现了类似红淡蛋白的蛋白质结合视网膜，可以用作样品的光感受性色素，2）cAMP和CGMP的浓度在白天且晚上很低，在夜间的特征很低，在恒定黑暗（DD）的状态（DD）和3）中的protein kinase canse can and base cass case case and case case case case case c的1次较低。在果蝇：1）两个突变体中，“ toki”和“ ritsu”是新隔离的，其昼夜节律与野生型果蝇不同。分析了突变体的遗传和时间生物学特征。此外，使用增强剂陷阱方法分离出一条没有表现出令人信服的昼夜节律周期性的突变线。2）复合眼似乎不充当生物体生物时钟系统的光转导系统。3。 Other animals :1) Even a small piece of the Newt pineal gland left, can serve as synchronizer of the circadian rhythm, b … More ut not as generator of the rhythm.2) The melatonin in the eyes of Quail shows a convincing rhythm in an light/dark cycle, which can be involved in the rhythmic cell division of retinal pigment epithelial cell, and3) The C3H mouse genetically lacking rod cell在白光刺激时，褪黑激素合成的抑制速率比正常CBA小鼠的抑制率少于正常的CBA小鼠的抑制率，其差异可能归因于CBA小鼠中的Rhodopsin的存在。4。通过酶 - 连接的免疫探测测量了哺乳动物昼夜节律时钟中摄入核核核的数量。阐明了事实之后：1）在光线刺激时，血管活性肠肽（VIP）改善并增加了胃蛋白释放的肽，而两个petides dld均未在DD.2中表现出昼夜节律。 SS的mRNA量在CT 0时上升到最大值，并降至12时。

项目成果

Tominaga, K., K. Shinohara, C. Fukuhara & S. -I. T. Inouye: "Circadian rhythms of vasopressin-immunoreactivity in the suprachiasmatic nucleus of the rat" Neuroscience Res. Suppl.16. S154 (1991)

富永，K.，K.筱原，C.福原

K.Hasunuma: "Nucleoside triphosphate (NTP)ーbinding proteins and endogenous ADPーribosyl transferase in <Neurospora>___ー <crassa>___ー." Plant and Cell Physiology.

K.Hasunuma：“核苷三磷酸 (NTP) - 结合蛋白和内源 ADP - 核糖基转移酶在 <Neurospora>___ - <crassa>___ - 植物和细胞生理学中。”

蓮沼 仰嗣: "植物細胞で信号はどう伝わるか:特集へのアプロ-チ" 遺伝. 44(1). 10 (1990)

Yotsugu Hasunuma：“信号如何在植物细胞中传递：特刊的方法”遗传学 44(1) 10 (1990)。

田中舘 明博,長谷川 建治,富岡 憲治,正木 忠勝,千葉 喜彦,蒲澤 良男,相良 嘉一,(千葉 喜彦,高橋 清久編): "研究法" 時間生物学ハンドブック. 504-519 (1991)

Akihiro Tanakadate、Kenji Hasekawa、Kenji Tomioka、Tadakatsu Masaki、Yoshihiko Chiba、Yoshio Kamazawa、Yoshikazu Sagara，（由 Yoshihiko Chiba 和 Kiyohisa Takahashi 编辑）：“研究方法”时间生物学手册 504-519（1991）。

Yoshida, S., A. Kana-uchi, K. Hasunuma and T. Murayama: "Cloning and sequence of a Neurospora ras gene." XVIth Fungal Genet. Conf. Abstr.50. (1991)

Yoshida, S.、A. Kana-uchi、K. Hasunuma 和 T. Murayama：“Neurospora ras 基因的克隆和序列。”