Studies on carbohydrate structures of plasma membrane blycoproteins in relation to the loss of cell adhesion.

质膜糖蛋白碳水化合物结构与细胞粘附丧失相关的研究。

基本信息

批准号：
62580108
负责人：
FURUKAWA Kiyoshi
金额：
$ 0.96万
依托单位：
University of Tokyo
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1987
资助国家：
日本
起止时间：
1987 至 1988
项目状态：
已结题

项目摘要

The carbohydrate structures of plasma membrane glycoproteins prepared from tissue forming cells and from non-tissue forming cells have been determined. The results showed that various tissues of rat contain mainly biantennary complex-type and high-mannose type sugar chains with different amounts of tri- and tetraantennary complex-type ones, of sialic acid, of fucose, of -galactose, of bisecting N-acetylglucosamine and of lactosaminoglycan in a tissue specific manner. When two rat ascites hepatoma cells were analyzed in comparison to normal livers, they were primarily tri- and tetraantennary complex-type sugar chains and those with a repeating structure which are different from that of livers. However, one ascites hepatoma cells, AH414, which lose cell adhesion and are present as single cells, expressed fucosyl hybrid-type sugar chains in much larger amount than livers and the other ascites hepatoma cells, AH7974, which maintain partial intercellular adhesion.When mucin-type glycoprotei … More ns associated with plasma membranes of liver and two ascites hepatoma of rat were analyzed by using a low density WGA-Sepharose column to which sialyl mucin-type glycoproteins preferentially bind, AH414 cells showed the presence of highly sialylated glycoprotein(s) with clusted mucin-type sugar chains, which were detected but in much lesser amount in liver and AH7974 cells. The carbohydrate structures of them were turned out to be GalNAc, Gal 1-GalNAc and Gal 1-(GlcNAc 1-)GalNAc.The above results indicated that the appearance of hybrid-type sugar chains as well as highly sialylated mucin-type sugar chains in plasma membrane glycoproteins may be related to the loss of cell adhesion of originally tissue forming cells. The activities of galactosyltransferases that are involved in the synthesis of Asn-linked and mucin-type sugar chains in the transformed cells were high, which probably may reflect the increased synthesis of branched Asn-linked sugar chains and of highly sialylated clustered mucin-type glycoproteins. Less

已经确定了从组织形成细胞和非组织形成细胞制备的质膜糖蛋白的碳水化合物结构。和四乙酸，乳酸，乳酸的四抗复合物型，在与正常肝脏相比，分析了两个大鼠肝癌细胞时，以E分析了两个大鼠肝癌细胞，它们是原始的Tri -and Tri -and Tetraantraantennarary，以E的方式分析了两个大鼠肝癌细胞。具有的复合体类型是不同的时间。通过使用WGA-Sepharse柱与使用WGA-Sepharse色谱柱分析粘蛋白型糖蛋白糖蛋白糖蛋白糖蛋白蛋白...与肝膜相关的更多NS和两种大鼠的腹水肝瘤相关。 s）粘蛋白型糖链，在肝脏和AH7974细胞中被检测到的碳水化合物结构少得多。最初形成细胞的组织的丧失。分支的ASN连接糖链和高度溶解的聚集粘膜糖蛋白。