Morphological and physiological investigation of the neuromuscular junction in the recurrent laryngeal nerve palsy

喉返神经麻痹神经肌肉接头的形态学和生理学研究

基本信息

批准号：
62570781
负责人：
TAKEMOTO Shin
金额：
$ 1.47万
依托单位：
Saga Medical School
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1987
资助国家：
日本
起止时间：
1987 至 1989
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-62570781/
关键词：
recurrent laryngeal nerve misdirected reinnervation freezing injury neurorrhaphy vocal fold electromyogram motoneuron movement of vocal fold 再生痲痺延髓神経筋接合部嗄声

项目摘要

The purpose of this investigation was to clarify misdirected reinnervation after injury of the recurrent laryngeal nerve(RLN).1. Degeneration and regeneration processes, and reorganization of the motoneurons reinnervating the posterior cricoarytenoid muscle(PCA) after freezing injury of the unilateral RLN of the guinea-pig were observed. Most axons degenerated within 3 days and regenerative sprouting with growth cones was observed after 7 days. From between 2 to 6 months, the number of the motoneuron in the PCA gradually increased and the area occupied by regenerated neurons gradually expanded. Regenerated PCA motoneurons were found outside the PCA motoneuron area of the control. Target specific regeneration was incomplete even after freezing injury in which neurites were damaged but the basal lamina was not affected.2. The movement of the vocal fold, electromyogram of the internal laryngeal muscles and reorganization of the PCA motoneurons were observed after neurorrhaphy or freezing injury of the unilateral RLN of the cat. In neurorrhaphy cases, the vocal fold on the damaged side was fixed in the paramedian position and the activity pattern of the vocalis muscle and PCA differed from those of the normal side even after 9 months. Many regenerated PCA motoneurons existed outside the PCA motoneuron area of the control. These results suggested that the dysfunction of the vocal fold in the affected side was caused by misdirected reinnervation in the internal laryngeal muscles. In freezing injury cases, movement of the vocal fold and electromyogram returned to normal after 4 months, and a few regenerated motoneurons were found outside the normal PCA motoneuron area. It is suggested that the functions of the internal laryngeal muscles were practically normal, although a slightly misdirected reinnervation occurred.

这项调查的目的是澄清复发性喉神经损伤后误导的重新支配（RLN）.1。观察到豚鼠单侧RLN冻伤后，衰老和再生过程，运动神经元重新构造后粒状肌肌肉（PCA）。大多数轴突在3天内退化，并在7天后观察到与生长锥的再生发芽。在2到6个月之间，PCA中的运动神经元的数量逐渐增加，而再生神经元占据的区域逐渐扩大。在对照的PCA运动神经元区域外发现了再生PCA运动神经元。即使在冻结神经突受损但基底层不影响的损伤后，靶标特异性再生也不完整。2。在神经毛病或猫的单侧RLN的冻伤后观察到声带的运动，内喉内部肌肉的肌电图和PCA运动神经元的重组。在神经毛电的情况下，受损方面的声带折叠在秘书位置固定，即使在9个月后，Vocalis肌肉和PCA的活性模式也与正常侧的活动不同。许多再生PCA运动神经元存在于对照的PCA运动神经元区域之外。这些结果表明，受影响的一侧声带的功能障碍是由内喉内部肌肉的重新连接引起的。在冻结伤害病例中，声带和肌电图的运动在4个月后恢复正常，并且在正常的PCA运动神经元区域外发现了一些再生运动神经元。据建议，尽管发生了稍微误导的加重，但内喉内部肌肉的功能实际上是正常的。