Humoral Autoimmunity in Gravs' Ophthalmopathy

格拉夫斯眼病的体液自身免疫

基本信息

批准号：
62570528
负责人：
NONAKA Kyohei
金额：
$ 1.22万
依托单位：
Kurume University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1987
资助国家：
日本
起止时间：
1987 至 1989
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-62570528/
关键词：
Grves' Ophthalmopathy Humoral Immunity Western Blotting Retroperitoneal Connective Tissue Retroocular Connective Tissue Tissue Crossreactivity ウェスタンブロッテング抗外眼筋抗体 CHAPSO アフィニティーカラムクロマトグラフィー enzyme linked immunosorbent assay emzyme lin

项目摘要

To test the hypothesis that humoral autoimmunity is involved as pathogenesis of Graves' ophthalmopathy(GO) we investigated an antigen antibody axis as yet unknown. Organs prepared for antigen were human retroorbital connective tissue, retroperitoneal connective tissue, extra ocular, muscle, thyroid, liver, spleen and heart. Antibodies were used as serum, crude IgG and affinity purified IgG using CHAPSO-solubilized retoperitoneal connective tissue membrane as antigen. Reaction of antibody with antigen was detected by SDS polyacrylamide gel electro- phoresis followed by Western blotting combined with immunodetection.2 out of 15 cases with GO had an antibody against 54kDa membrane protein of retroperitoneal connective tissue. Whilst this antibody showed crossreactivity against membrane protein of retro orbital connective tissue and extraocular muscle, it's molecular weight was 29kDa. The reaction was very faint in the latter. 54kDa or 29kDa antigen did not exist in other organs tested. This antibody was not detected in 13 cases of 10 normals and I Hashimoto thyroiditis, I silent thyroiditis and I thyroid adenoma.We conclude I An immunologically identical antigenic determinant exist between retroperitoneal connective tissue, retroorbital connective tissue and extraocular muscle. 2 Membrane proteins on which this antigenic determinant exist differ in molecular weight, being 54kDa, 29kDa and 29kDa respectively. 3 Amount of 29kDa protein on extraocular muscle membrane is very little. 4 Other organs tested do not have these antigens. 5 Autoantibody against 54kDa protein is present in some of sera from patient with GO. Although significance of this antibody in pathogenesis of Go is not known at the present, this is a new potential antibody different from any antibodies ever found.

为了检验体液自身免疫与格雷夫斯眼病（GO）发病机制有关的假设，我们研究了尚不清楚的抗原抗体轴。制备抗原的器官是人眼眶后结缔组织、腹膜后结缔组织、眼外、肌肉、甲状腺、肝脏、脾脏和心脏。使用 CHAPSO 溶解的腹膜后结缔组织膜作为抗原，将抗体用作血清、粗制 IgG 和亲和纯化的 IgG。采用SDS聚丙烯酰胺凝胶电泳、Western blotting结合免疫检测检测抗体与抗原的反应。15例GO患者中，有2例检测到腹膜后结缔组织54kDa膜蛋白抗体。虽然该抗体对眼眶后结缔组织和眼外肌的膜蛋白表现出交叉反应性，但其分子量为 29kDa。后者的反应非常微弱。所测试的其他器官中不存在54kDa或29kDa抗原。在10例正常人以及I桥本甲状腺炎、I沉默甲状腺炎和I甲状腺腺瘤的13例中未检测到该抗体。我们得出结论I腹膜后结缔组织、眼眶后结缔组织和眼外肌之间存在免疫学上相同的抗原决定簇。 2 存在该抗原决定簇的膜蛋白的分子量不同，分别为 54kDa、29kDa 和 29kDa。 3 眼外肌膜上29kDa蛋白质含量很少。 4 所测试的其他器官没有这些抗原。 5 GO 患者的一些血清中存在针对 54kDa 蛋白的自身抗体。尽管目前尚不清楚该抗体在 Go 发病机制中的重要性，但这是一种不同于迄今为止发现的任何抗体的新的潜在抗体。