Ultrastructural and physiological studies on the function of pituitary intermediate lobe in mice.

小鼠垂体中叶功能的超微结构和生理学研究。

基本信息

批准号：
62540568
负责人：
KOBAYASHI Yasuo
金额：
$ 1.28万
依托单位：
Okayama University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1987
资助国家：
日本
起止时间：
1987 至 1989
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-62540568/
关键词：
Mouse Pituitary intermediate lobe Electron microscope Morphometry Polydipsia-polyurea Urea 浸透圧多飲

项目摘要

The present studies demonstrated a new fact that oral urea administration resulted in hypersecretion of pituitary intermediate lobe(PIL) cells in mice. Oral urea is the third factor, besides our previous studies of dietary Na-deficiency (1974,1976) wid of experimental polydipsia (1984), that stimulates PIL cells in fine structure. The outline of the ultrastructural and morphometric studies is described as follows:(1) Alterations in PIL cells of suckling mice fed a liquid diet supplemented with 1% urea. Suckling dams (day 12-17 of lactation) were fed with either a liquid diet (11.5% powdered milk) or the liquid diet containing 1% urea. Morphometric study resulted in the hypersecretion in PIL cells of dams being given the urea-containing liquid diet more significantly than that of the liquid diet control.(2) Alterations in PIL cells of male mice fed a liquid diet supplemented with 1% urea. Young adult male mice were fed with the liquid diet containing 1% urea for 10 days in order to ascertain whether oral urea could stimulate PIL cells not only in lactating dams but also in male mice. The morphometric analysis showed the result that oral urea stimulated significantly the secretory activity of PIL cells in male mice.(3) Alerations in PIL cells of male mice given the pelleted food containing urea in the concentration of 6%, 12% and 24% for 14 days. Morphometric result revealed the increment of segretion activity in PIL cells of mice given urea- containing pellets in a dose dependent manner. Water consumption also increased in parallel to the urea concentrations adopted. These results are interpreted that oral urea stimulation of PIL cells is through a mechanism of either (1)attenuation of hypothalamic inhibitory control over PIL cells or (2)an involvement of PI hormones in the excretion of excess urea, or (3) hyponatremia caused by high blood urea concentration.

本研究表明，口服尿素给药导致小鼠中垂体中间叶（PIL）细胞的分泌。口服尿素是第三个因素，除了我们先前对饮食NA缺乏症的研究（1974,1976）的实验多磷酸（1984），还刺激了PIL细胞的精细结构。超微结构和形态学研究的轮廓如下：（1）喂食补充1％尿素的液体饮食的PIL细胞的改变。用液体饮食（11.5％粉末牛奶）或含有1％尿素的液体饮食喂食哺乳水坝（泌乳的第12-17天）。形态计量学研究导致在大坝的PIL细胞中的过度分泌，含尿素的液体饮食比液体饮食控制的含量更明显。（2）喂食含有1％尿素的液体饮食的雄性小鼠的PIL细胞改变。将年轻的成年雄性小鼠用含有1％尿素的液体饮食喂食10天，以确定口服尿素是否可以刺激PIL细胞不仅在泌乳大坝中，而且还可以在雄性小鼠中刺激。形态分析表明，口服尿素在雄性小鼠中显着刺激了PIL细胞的分泌活性。（3）雄性小鼠的PIL细胞中含有含有尿素的尿素浓度为6％，12％和24％的雄性小鼠的PIL细胞，为14天。形态测定结果表明，以剂量依赖性方式给予尿素的小鼠的PIL细胞中脱生活性的增加。用水量也与采用的尿素浓度并行增加。这些结果解释说，口服尿素刺激PIL细胞是通过（1）（1）下丘脑对PIL细胞的抑制性控制的机制，或（2）PI激素参与过量尿素的排泄，或（3）低血液尿素浓度引起的低钠血症。