A study on the role of humoral antibody against Bacteroides gingivalis.

牙龈拟杆菌体液抗体作用的研究。

基本信息

批准号：
60570858
负责人：
OKUDA Katsuji
金额：
$ 1.02万
依托单位：
Tokyo Dental College
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1985
资助国家：
日本
起止时间：
1985 至 1986
项目状态：
已结题

项目摘要

Bacteroide gingivalis has been implicated in many cases of adult periodontitis. Elevation of humoral immune responses to B. gingivalis in subjects with adult periodontitis has been reported in our laboratory. In the present study, we investigated the relationship between bactericidal activity for B. gingivalis in human serum and IgG antibody levels to the organisms.Susceptibility of B. gingivalis strains to the complement mediated bactericidal activity of human serum was examined under anaerobic conditions. Serum containing high concentrations of IgG antibody to the microorganisms enhanced the bactericidal activity. No enhancement was found in serum which did not contain the specific antibody or in serum absorbed with intact cells. The sensitivity to the killing by pooled serum differed among B. gingivalis strains. Cells of B. gingivalis activated the pooled human serum complement not only through the classical pathway but also through an alternative pathway. It was found that the susceptibility of B. gingivalis to the bactericidal activity was classical pathway-dependent.We reported that gingival crevicular fluid contained both appreciable amounts of immunoglobulins containing IgG antibody to B. gingivalis and a complement system. These findings may support the argument that the specific antibody and complement in the gingival crevice are important determinants of host resistance to the micro-organism in vivo.

牙龈拟杆菌与许多成人牙周炎病例有关。我们的实验室报告称，患有成人牙周炎的受试者对牙龈芽胞杆菌的体液免疫反应升高。在本研究中，我们研究了人血清中牙龈芽孢杆菌的杀菌活性与该生物体的IgG抗体水平之间的关系。在厌氧条件下检查了牙龈芽孢杆菌菌株对人血清补体介导的杀菌活性的敏感性。血清中含有高浓度的IgG抗体，对微生物增强了杀菌活性。在不含特异性抗体的血清或被完整细胞吸收的血清中未发现增强。不同牙龈芽胞杆菌菌株对混合血清杀灭的敏感性不同。牙龈芽胞杆菌细胞不仅通过经典途径而且还通过替代途径激活汇集的人血清补体。结果发现，牙龈芽孢杆菌对杀菌活性的敏感性是经典途径依赖性的。我们报道龈沟液中含有大量的免疫球蛋白和补体系统，其中免疫球蛋白含有针对牙龈芽孢杆菌的IgG抗体。这些发现可能支持这样的论点：牙龈缝隙中的特异性抗体和补体是宿主体内微生物抵抗力的重要决定因素。