Pathogenesis of myonecrosis following cerebral vasospsm

脑血管痉挛后肌坏死的发病机制

• 批准号: 60570665
• 负责人: YAMASHIMA Tetsumori
• 金额: $ 0.96万
• 依托单位: University of Kanazawa
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1985
• 资助国家: 日本
• 起止时间: 1985 至 1987
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-60570665/
• 关键词: Subarachnoid hemorrhage; Meningitis; Putaminal hemorrhage; Cerebral vasospasm; Myonecrosis; Dog; Epinephrine; 電子顕微鏡; 脳血管攣縮; イヌ

Myonecrosis following cerebral vasospasm associated with subarachnoid hemorrhage, meningitsis and trans-sylvian surgery was ultrastructurally studied. The basic feature of myonecrosis was dissolution of myofilaments with resultant fine granular or filamentous material. The disintegrating cytoplasm often contained numerous glycogen granules, dense bodies, autophagic vacuoles and myelin-like membranous bodies. A well-developed sarcoplasmic reticulum was preserved despite myofilament dissolution, while mitochondria showed marked swelling. The nuclei showed either dilution of chromatin or pyknotic change. The basal lamina was remarkably thickened and maintained an irregular outline of the necrotic smooth muscle cells. Enlarged intercellular space contained abundant cellular debris, vesicular structures and connective tissue fibers. Furthermore, myonecrosis following the injection of epinephrine into the canine chiasmatic cistern was studied. Microscopically, the circle of Willis showed coagulation necrosis and fibrosis of the media. The fine structure of myonecrosis was characterized by six dynamic chenges of vacuolation, dissolution of myofilaments, focal cytoplasmic necrosis, fragmentation, coagulation necrosis and intercellular fibrosis. Despite a simple experimental procedure, the present models disclosed myonecrosis with a marked similarity to humans and contained all of the previously reported ultrstructural features of experimental myonecrosis.

与蛛网膜下腔出血，脑膜炎和跨囊体手术有关的脑血管痉挛后的肌瘤症进行了超微结构研究。肌表皮症的基本特征是肌丝溶解，导致细粒或丝状物质。崩解的细胞质通常包含许多糖原颗粒，致密的身体，自噬液泡和髓磷脂样膜体。尽管溶解了丝丝，但仍保留了发达的肌浆网，而线粒体显示出明显的肿胀。细胞核显示染色质或膨化变化的稀释。基底层被显着增厚，并保持了坏死平滑肌细胞的不规则轮廓。扩大的细胞间空间包含丰富的细胞碎片，水泡结构和结缔组织纤维。此外，还研究了肾上腺素注射后的肌分布。从显微镜上讲，威利斯的圆显示了培养基的凝血坏死和纤维化。肌症的细胞结构的特征是六个动态液泡，肌细胞溶解，局灶性细胞质坏死，碎裂，凝结坏死和细胞间纤维化。尽管进行了简单的实验程序，但本模型仍披露了与人类明显相似性的肌瘤作用，并包含了所有先前报道的实验性肌发电的超结构特征。

项目成果

Tetsumori Yamashima: Neurosurgery. 16. 546-553 (1985)

山岛哲守：神经外科。

Tetsumori Yamashima: "Fine structure of myonecrosis following epinephrine-induced cerebral vasospasm" Neurochirurgia. 30. 29-34 (1987)

Tetsumori Yamashima：“肾上腺素引起的脑血管痉挛后肌坏死的精细结构”神经外科。

Tetsumori Yamashima: "Meningothelial rosettes in the canine subarachnoid space" Acta Neuropathologica (Berlin). 66. 223-232 (1985)

Tetsumori Yamashima：“犬蛛网膜下腔中的脑膜玫瑰花结”Acta Neuropathologica（柏林）。

Tetsumori Yamashima: Acta Neuropathologica(Berlin). 66. 223-232 (1985)

山岛哲森：神经病理学学报（柏林）。

Tetsumori Yamashima: Journal of Neurology. 233. 348-357 (1986)

山岛哲森：神经病学杂志。