'Cradle to the grave' life histories of every bacterium link individual metabolic activity to population activity

每种细菌的“从摇篮到坟墓”的生命史将个体代谢活动与群体活动联系起来

• 批准号: 22K18368
• 负责人: UTADA ANDREW
• 金额: $ 16.56万
• 依托单位: University of Tsukuba
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Research (Pioneering)
• 财政年份: 2022
• 资助国家: 日本
• 起止时间: 2022-06-30 至 2025-03-31
• 项目状态: 未结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22K18368/
• 关键词: heterogeneity; metabolism; NanoSIMS; Single-cell tracking; Metaboloic activitity; Bacteria; Heterogeneity

In the past year, we have started by finding an appropriate research scientist. We have begun to identify a set of bacteria to use that are genetically tractable and widely applicable. This list currently includes Escherichia coli and Pseudomonas aeruginosa. We have focused on developing the two major thrusts of our targeted work: single cell tracking in confined conditions and the nano secondary ion mass spectrometry (nanoSIMS) process. We have already begun to optimized our cell tracking using publicly available machine learning algorithms combined with custom designed microfluidic chambers.Simultaneously, we have developed microfabrication methods to use with hard glass substrates. We plan to combine these results soon to use with the local nanoSIMS facility.

在过去的一年中，我们从找到合适的研究科学家开始。我们已经开始确定一组可使用的细菌，这些细菌在遗传上可牵引且广泛适用。此列表当前包括大肠杆菌和铜绿假单胞菌。我们专注于开发目标工作的两个主要推力：在狭窄条件下的单细胞跟踪和纳米次级离子质谱（Nanosim）过程。我们已经开始使用公开可用的机器学习算法与定制设计的微流体室进行了优化的细胞跟踪。类似地，我们开发了与硬玻璃基板一起使用的微加工方法。我们计划很快将这些结果结合起来，以与当地的纳米Sims设施一起使用。