Development of hybrid vascular grafts with accelerated healing due to activated autologous cytokines

由于激活的自体细胞因子而加速愈合的混合血管移植物的开发

基本信息

批准号：
09680865
负责人：
TOMIZAWA Yasuko
金额：
$ 2.3万
依托单位：
Tokyo Women's Medical University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1997
资助国家：
日本
起止时间：
1997 至 1998
项目状态：
已结题

项目摘要

Basic fibroblast growth factor (bFGF) is one of the strongest angiogenic agent, and affects all cell types involved in wound healing. The distribution of bFGF is widespread, because it plays an important role in regulating cell proliferation and mitosis. Angiogenesis by bFGF is important for endothelialization in vascular prostheses and is significant from shortly after implantation. Lack of angiogenic factors in the vascular graft wall may cause for unhealing neointima. Collateral formation in ischemic heart disease is induced by angiogenic factors including bFGF.We hypothesized that tissue-fragmented (TF-) grafts are rich of angiogenic activity from shortly after implantation and it ability may work for collateral formation to the ischemic area when it is used for a small diameter vascular prosthesis in the coronary artery position. We developed a TF-graft and observed the healing process including angiogenesis and endothelialization. We designed the vascular graft which is made of fabric and tissues with cells. And, this is "Hybrid". Proliferation and mitosis of endothelial cells were active in the graft wall 5 days after implantation. The full thickness of the intima was organized at 7 days. These results demonstrated that endogenous bFGF is important for endothelialization due to angiogenesis in fabric vascular prostheses, whereas thrombus and infection might have a negative effect. It might be possible to have patent grafts in the coronary position, if we could have fine basic small-diameter fabric prosthesis with good handling characteristics, excellent antithrombogehicity.

碱性成纤维细胞生长因子（BFGF）是最强的血管生成剂之一，并影响与伤口愈合有关的所有细胞类型。 BFGF的分布很普遍，因为它在调节细胞增殖和有丝分裂中起着重要作用。 BFGF的血管生成对于血管假体的内皮化很重要，并且植入后不久就很重要。血管移植壁缺乏血管生成因子可能导致新的新神经。包括BFGF在内的血管生成因素诱导缺血性心脏病的副作用。我们假设，植入后不久，组织碎片（TF-）移植物具有富含血管生成活性，并且IT能力可能在小型直径的小直径高管血管中的缺血区域的核心形成中起作用。我们开发了一种TF移植物，并观察了包括血管生成和内皮化的愈合过程。我们设计了由织物和细胞组织制成的血管移植物。而且，这是“混合”。植入后5天，内皮细胞的增殖和有丝分裂在移植物壁上活跃。内膜的全厚度在7天内组织了。这些结果表明，由于织物血管假体的血管生成，内源性BFGF对于内皮化很重要，而血栓和感染可能会产生负面影响。如果我们可以拥有具有良好的操纵特性，出色的抗凝血剂，则有可能将专利移植物处于冠状动脉位置。