Search for Novel Anti-tumor Constituents from Plant Sources and Their Drug Development

从植物中寻找新型抗肿瘤成分及其药物开发

• 批准号: 09672173
• 负责人: FUKUKAWA Hiroshi
• 金额: $ 1.86万
• 依托单位: Meijo University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09672173/
• 关键词: Rutaceae; Leguminosae; Guttiferae; Avicenniaceae; coumarins; alkaloids; xanthones; biological activity; フラボノイド; Citrus; Clausena; Glycosmis; Garcinia; カルバゾール; アクリドン; キナゾロン; Calophyllum

In the search of novel anti-tumor constituents from plant sources, many kinds of novel acridone-, carbazole-, quinolone-, and quinazoline-alkaloids, and xanthones, coumarins, fulalvonoids, and phenylpropanoids were isolated and their structures were established by spectrometric and synthetic methods. Further, several types of biological activities were also tested for the compounds isolated in this project.1. Families and genus of plant sources used in this project :(1) Plants of Rutaceae : plants of genus Murraya, Clausena, Glycosmiss, Boronia, Micromelum(2) Plants of Leguminosae : plants of genus Dalbergia, Milletia, Derris(3) Plant of Guttiferae : plants of genus Calophyllum, Garcinia(4) Plant of Avicenniaceae : plants of genus Avicennia2. Assays of Biological Activities(1) Assay of Anti-tumor Promoting Effects : 7-methoxycoumarins, xanthones, Phenylpropanoids(2) Assay of the Activity of Induction of HL-60 Cell Differentiation : acridone alkaloids(3) Assay of Anti-proliferative Effects on Several Cancer Cell Lines : acridone alkaloids(4) Assay of Inhibitory Effects of Nitric Oxide Generation : coumarins

为了搜索植物来源的新型抗肿瘤成分，许多新型的acridone-，carbazole-，quinolone-和quinazoline-alkaloids以及Xanthones，Coumarins，fulalvonoids和fulalvonoids和苯基类动物均被隔离，其结构是通过光谱和组合方法建立的。此外，还测试了该项目中分离出的几种类型的生物活性。1。 Families and genus of plant sources used in this project :(1) Plants of Rutaceae : plants of genus Murraya, Clausena, Glycosmiss, Boronia, Micromelum(2) Plants of Leguminosae : plants of genus Dalbergia, Milletia, Derris(3) Plant of Guttiferae : plants of genus Calophyllum, Garcinia(4) Plant of Avicenniaceae : Avicennia2属的植物。生物学活性的测定（1）抗肿瘤促进作用的测定：7-甲氧基木蛋白，Xanthone，苯基丙糖苷（2）HL-60细胞分化的诱导活性的测定：阿环酮碱基（3）对几种癌细胞的影响（3）对抗癌细胞的影响（3）癌细胞的效应：cariDONE氧化物（4）（4 ：香豆素

项目成果

Satoru Kawaii: "The Antiproliferative Effect of Acridone Alkaloids on Several Cancer Cell Lines"J. Nat. Prod.. 62. 587-589 (1999)

Satoru Kawaii：“吖啶酮生物碱对几种癌细胞系的抗增殖作用”J。

Akira Murakami: "1,1-Dimethylallylcoumarins Potently Supress both Lipopoly- saccharide- and Interferon- -induced Nitric Oxide Generation in Mouse Macrophage RAW 264.7 Cells."Booing. & Med. Chem. Lets.. 10. 59-62 (2000)

Akira Murakami：“1,1-二甲基烯丙基香豆素可有效抑制脂多糖和干扰素诱导的小鼠巨噬细胞 RAW 264.7 细胞中一氧化氮的产生。”嘘声。

Chihiro Ito: "Chemical Constituents of Glycosmis pentaphylla.Isolation of A Novel Naphthoquinone and A New Acridone Alkaloid."Chem.Pharm.Bull.. 47. 1579-1581 (1999)

Chihiro Ito：“Glycosmis pentaphylla 的化学成分。新型萘醌和新吖啶酮生物碱的分离。”Chem.Pharm.Bull.. 47. 1579-1581 (1999)

Akira Murakami: "Identification of Coumarins from the Fruit of Citrus Hystrix DC. as Inhibitors of Nitric Oxide Generation in Mouse Macrophage RAW264.7 Cills"J. Agr. & Food Chem.. 47. 333-339 (1999)

Akira Murakami：“鉴定柑橘 Hystrix DC 果实中的香豆素作为小鼠巨噬细胞 RAW264.7 Cills 中一氧化氮生成的抑制剂”J。

Chihiro Ito: "Anti-tumor-promoting effects of 8-substituted 7-methoxyxoumarins on Epstein-Barr virus activation assay"Cancer Letts. 138. 87-92 (1999)

Chihiro Ito：“8-取代的 7-甲氧基香豆素对 Epstein-Barr 病毒激活测定的抗肿瘤促进作用”Cancer Letts。