Regulatory Mechanism of Nitric Oxide on Olfactory Mucosa Secretion-Elucidation of Olfactory Mucosa Secretion Abnormality at Time of Olfactory Disturbance-

一氧化氮对嗅觉粘膜分泌的调节机制-嗅觉障碍时嗅粘膜分泌异常的阐明-

基本信息

批准号：
09671763
负责人：
HARUNA Shinichi
金额：
$ 1.92万
依托单位：
THE JIKEI UNIVERSITY SCHOOL OF MEDICINE
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1997
资助国家：
日本
起止时间：
1997 至 1999
项目状态：
已结题

项目摘要

1. An animal experimental model of sinusitis was prepared using New Zealand White rabbits and bacteria (Streptococcus pneumoniae serotype 3, and Staphylococcus aureus V8 ; 107-109 CFU). Beginning from the fifth day after inoculation of the bacteria, bowman glands, the mucosal epithelium and the cell surface were found to have become ConA-positive and SNA-negative. In rabbits whose inflammatory symtoms showed improvement after 12 weeks or more post-inoculation when observed under a light microscope, Bowman glands, the mucosal epithelium and the cell surface were found to have become SNA-positive while Con A had become negative. These results represented a return to the normal staining pattern.2. In patients who had undergone surgery for chronic sinusitis accompanied by olfactory disturbance, the olfactory mucosa showed strong positivity for MAA on the cell surface and Bowman glands. Moreover, Bowman glands, supporting cells and the cell suface stained positively for SNA, PNA and UEA-1, … More and also for PNA after treatment with sialidase. In addition, there was an increase in the viscosity of the mucus and increased secretion of sialic acid. At several months after the surgery, the olfactory mucosa of some of the patients in whom the chronic sinusitis had been cured showed reduced positivity (I.e., fainter staining intensity) for MAA, PNA and UEA-1 in bowman glands. It was surmised that there had been normalization of the viscosity of the mucus and the secretion of sialic acid.3. An apparatus for measuring NO by a chemoluminescence technique was used to determine the concentration of NO in air exhaled through the nose and through the mouth. The measured values were compared to examine whether or not there was a difference due to the presence or absence of olfactory disturbance caused by chronic sinusitis. The results did not find a clear difference. When an immunohistochemical study by means of NOS was carried out in relation to the two cases of the presence or absence of olfactory disturbance, the cilia, goblet cells and glandular tissue were positive when iNOS antibody was used, while perivascular positivity was observed when cNOS antibody was employed. Again, no clear difference was found between the two cases.4. An attempt was made at secondary culture of the olfactory mucosa of the rabbit by means of reconstitution of the olfactory mucosa epithelium on a base of firoblasts and cerebral astroglia. However, as of this writing, we have not been successful in establishing a stable cell line of olfactory cells in culture medium. Less

1。鼻窦炎的动物实验模型是使用新西兰白兔和细菌（肺炎链球菌血清型3和金黄色葡萄球菌V8; 107-109 CFU）制备的。从接种细菌后的第五天开始，发现鲍曼腺，粘膜上皮和细胞表面已成为CONA阳性和SNA阴性。在12周或更多后接种后炎症性症状的兔子中，当在光学显微镜下观察到鲍曼腺时，粘膜上皮和细胞表面已被发现变为SNA阳性，而CON A A A已变为阴性。这些结果代表了恢复正常染色模式2。在接受嗅觉灾难完成的慢性鼻窦炎手术的患者中，嗅觉粘膜在细胞表面和鲍曼腺上表现出强大的MAA阳性。此外，鲍曼腺，支撑细胞和细胞表面对SNA，PNA和UEA-1的阳性染色，以及用唾液酸酶处理后的PNA。此外，粘液的粘度和唾液酸分泌的增加也有所增加。手术后的几个月，一些慢性鼻窦炎治愈的患者的嗅觉粘膜显示出Bowman腺体中MAA，PNA和UEA-1的积极性降低（即失败染色强度）。据推测，粘液的粘度正常化和唾液酸的分泌。3。通过化学发光技术测量NO的一种设备用于确定鼻子耗尽的空气中NO的浓度。将测量值与检查是否由于慢性鼻窦炎引起的嗅觉灾难是否存在差异。结果没有发现明显的差异。当通过使用嗅觉灾难的两种情况或不存在的两种情况下，进行了通过NOS进行免疫组织化学研究时，当使用iNOS抗体时，纤毛，杯状细胞和腺组织是阳性的，而使用牙齿抗体抗体呈肌血管抗体阳性。同样，两种情况之间没有发现明显的差异。4。通过在弱势粘膜上皮的基础上重新建立了兔子和脑星形胶质细胞的嗅觉粘膜嗅觉粘膜的次要培养物进行了尝试。但是，在撰写本文时，我们尚未成功在培养基中建立嗅觉细胞的稳定细胞系。较少的