New chloroplast genes involved in assembly of photosystem 1 complex.

新叶绿体基因参与光系统 1 复合体的组装。

• 批准号: 09640774
• 负责人: TAKAHASHI Yuichiro
• 金额: $ 1.6万
• 依托单位: Okayama University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09640774/
• 关键词: photosynthesis; photosystem 1; ycf3; ycf4; molecular assembly; Chlamydomonas reinhardtii; chloroplast transformation; molecular chaperone; 葉緑体; Ycf4遺伝子

The photosystem 1 drives light-induced electron transfer reaction from plastocyanin to ferredoxin and consists of more than ten subunits and over one hundred cofactors. The biogenesis of this complex requires cooperative assembly of these components.In the present study, the chloroplast genes ycf3 and ycf4 from the green alga Chiamydomonas reinhardtii have been characterized. Using biolistic transformation, ycf4 or were disrupted with a chioroplast selectable marker cassette. Transformants lacking ycf4 or ycf3 were unable to grow photoautotrophically and were deficient in photosystem I activity. Western blot analysis showed that the photosystem I (PSI) complex does not accumulate stably in thylakoid membranes of these transformants. Ycf4 and Ycf3 were localized on thylakoid membranes but not stably associated with the PSI complex and accumulated to wild type levels in mutants lacking PSI.RNA blot hybridizations showed that transcripts of psaA, psaB and psaC accumulate normally in these mutants and use of chimeric reporter genes revealed that Ycf3 is not required for initiation of translation of psaA and psaB mRNA.Our results indicate that Ycf3 and Ycf4 are required for stable accumulation of the PSI complex.

光系统1驱动光诱导的从质体蓝素到铁氧还蛋白的电子转移反应，由十多个亚基和一百多个辅助因子组成。该复合物的生物发生需要这些成分的协同组装。在本研究中，来自绿藻莱茵衣藻的叶绿体基因 ycf3 和 ycf4 已得到表征。使用基因枪转化，用叶绿体选择标记盒破坏ycf4或ycf4。缺乏 ycf4 或 ycf3 的转化体无法光自养生长并且缺乏光系统 I 活性。蛋白质印迹分析表明，光系统 I (PSI) 复合物在这些转化体的类囊体膜中不能稳定积累。 Ycf4 和 Ycf3 位于类囊体膜上，但与 PSI 复合体不稳定相关，并且在缺乏 PSI 的突变体中积累到野生型水平。RNA 印迹杂交表明 psaA、psaB 和 psaC 的转录本在这些突变体中正常积累，并且使用嵌合报告基因结果表明，Ycf3 不是 psaA 和 psaB mRNA 翻译起始所必需的。我们的结果表明，Ycf3 和 Ycf4 是 psaA 和 psaB mRNA 稳定积累所必需的。 PSI 复合体。

项目成果

Y.Takahashi, F.Taguchi, Y.Takahashi and K.Satoh: "Chloroplast-encoded small subunits of the three multiprotein complexes in photosynthetic electron transport" J.Plant Research. 111. 101-111 (1998)

Y.Takahashi、F.Taguchi、Y.Takahashi 和 K.Satoh：“光合电子传递中三种多蛋白复合物的叶绿体编码小亚基”J.Plant Research。

E.Boudreau, Y.Takahashi et al.: "The chloroplast ycf3 and ycf4 open reading frames of Chlamydomonas reinhardtii are required for the accumulation of the photosystem I complex" The EMBO Journal. 16・20. 6095-6104 (1997)

E. Boudreau、Y. Takahashi 等人：“莱茵衣藻的叶绿体 ycf3 和 ycf4 开放阅读框是光系统 I 复合体积累所必需的”The EMBO Journal 16・20 (1997)。

Y.Takahashi: "Chloroplast-encoded small subunits of the three multiprotein complexes in photosynthetic electron transport" J.Plant Research. 111. 101-111 (1998)

Y.Takahashi：“光合电子传递中三种多蛋白复合物的叶绿体编码的小亚基”J.Plant Research。

A.Hatano,Y.Takahashi: "Characterization of Chlanydomonas tranformants with impaired processing of a precursor D1 and oxygen-evolving activity of photosystem II" Proceedings of XIth International Congress of Photosynthsis. (印刷中).

A. Hatano，Y. Takahashi：“前体 D1 加工和光系统 II 释氧活性受损的衣单胞菌转化体的表征”第十一届国际光合作用大会论文集（正在出版）。

E.Boudreau,Y.Takahashi et al.: "The chloroplast ycf3 and ycf4 open reading frames of Chlamydomonas reinhardtii are required for the accumulation of the photosystem I complex" The EMBO Journal. 16. 6095-6104 (1997)

E.Boudreau,Y.Takahashi 等人：“莱茵衣藻的叶绿体 ycf3 和 ycf4 开放阅读框是光系统 I 复合体积累所必需的”EMBO 杂志。