Strain Establishment, Storage, and Application of the Fungivorous Nematode, Aphelenchus avenae.

食真菌线虫燕麦滑刃线虫的菌株建立、储存和应用。

• 批准号: 09460030
• 负责人: ISHIBASHI Nobuyoshi
• 金额: $ 3.65万
• 依托单位: SAGA UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09460030/
• 关键词: fungivorous nematodes; Aphelenchus avenae; soil conditioner; biological control; soil-borne fungal diseases; mass-production; long-term preservation; application; 土壌妨害防除; 糸状菌選好性; 系統確立; 整剤化; 混合施用; 総合防除; 産業廃棄物

1) Strain establishment : 39 isolates were mostly collected from Kyushu. Others were from Okinawa, Kanto, and Tohoku districts. These isolates were divided into 5 groups according to their host fungi preference, temperature optima for reproduction, and male occurrence. However, PCR-RLFP and PCR-RAPD analyses did not show any relevance to these preference or male occurrence. Only esterase isoenzyme phenotypes had a slight relevance to these traits. Host/temperature preferences may have stemmed from an adaptation or acclimatization.2) Mass-production and long-term preservation The NEMATODES are mass-produced by feeding fungal hyphae on solid substrates of various industrial vegetable wastes in different combinations. The mixed medium of vegetable and animal wastes greatly enhanced the NENATODE multiplication. Addition of used green tea or tea plant debris (30%) is essential component of the medium. From initial 200 nematodes, 1.2 x 10ィイD16ィエD1 were harvested 20 days after inoculation on … More the mixed media. NEMATODES are also mass-produced on sponge substrates permeated with PDA solution as a nutrient source for the host fungus. The highest multiplication 1.1 x 10ィイD16ィエD1 was recorded on 20 g substrates 30 days after inoculation. The produced NEMATODES are put in a hollow pellet made from used medium that was spent for production of NEMATODES. The pellet containing NEMATODES 20,000 - 100,000 per pellet are placed in a drying chamber for about 30 hr. After the NEMATODES entered steady anhydrobiolic state, they can be stored in a room at ambient temperature for more than one year without loosing the viability.3) Application : NEMATODES can be applied widely in farming crops for biological control of soil-borne fungal diseases of plants, and for integrated control of soil pests other than fungi by mixed application with other biocontrol agents. Dried pellets containing NEMATODES (20,000/pellet) are applied to a seedling pot with 200-300 ml soil for germination of seeds or propagation of seedlings or to field soil at the base of plants.4) Patent application : Patents were applied to Japan, USA, EC, and Australia on the title "Mass-production, long-term preservation of the fungivorous nematode, Aphelenchus avenae, and uses thereof". In Japan and Australia, they were approved, and now on-going in USA and European Communities. Less

1) 菌株建立： 39 个分离株主要来自九州，其他来自冲绳、关东和东北地区。这些分离株根据其寄主真菌偏好、繁殖最适温度和雄性发生情况分为 5 组。 -RLFP 和 PCR-RAPD 分析没有显示与这些偏好或雄性发生有任何相关性，只有酯酶同工酶表型与这些性状可能源于宿主/温度偏好。 2) 大规模生产和长期保存 线虫是通过在各种工业蔬菜废物的固体基质上以不同的组合喂养真菌菌丝来大量生产的，植物和动物废物的混合培养基大大增强了线虫的繁殖。添加用过的绿茶或茶树残渣 (30%) 是培养基的重要组成部分 从最初的 200 条线虫中，1.2 x 。接种 20 天后，在混合培养基上收获 10D16D1，并在浸有 PDA 溶液作为宿主真菌营养源的海绵基质上大量生产，在 20 g 基质上记录了最高倍增率 1.1 x 10D D16。接种后几天，将产生的线虫放入由用过的培养基制成的空心颗粒中。用于生产 NEMATODES 的颗粒被放置在干燥室中约 30 小时，当 NEMATODES 进入稳定的脱水状态后，它们可以在环境温度下储存在房间中超过 1 个。 3) 应用：NEMATODES可广泛应用于农作物，用于植物土传真菌病害的生物防治，以及用于综合防治真菌以外的土壤害虫，与其他生物防治剂混合施用，将含有线虫的干燥颗粒（20,000粒/粒）施用于装有200-300毫升土壤的育苗盆中，用于种子发芽或幼苗繁殖或施用于田间土壤。 4) 专利申请 : 已向日本、美国、欧共体、澳大利亚申请专利，标题为“大量生产、长期保存食真菌线虫、燕麦滑刃线虫及其用途。在日本和澳大利亚，它们已获得批准，目前正在美国和欧洲共同体进行。少

项目成果

Ali.Md.Rustom and N.Ishibashi: "genetic diversity among isolates of fungivorous nematode,Aphelenchus avenae,from Kyushu and Okinawa in Japan." Japanese Journal of Nematology. 29(発表予定).

Ali.Md.Rustom 和 N.Ishibashi：“来自日本九州和冲绳的食真菌线虫分离株的遗传多样性”，《日本线虫学杂志》29（待出版）。

Ishibashi,Nobuyoshi: "Behaviour : Behaviour of Entomopathogenic Nematodes" Hewitt Publisher UK, 800-1000 (1999)

Ishibashi, Nobuyoshi：“行为：昆虫病原线虫的行为” Hewitt Publisher UK，800-1000 (1999)

Ishibashi, N.: "Mineralization of nutrients : The role of soil invertebrates ; Protozoans, Enchytraeidae, and Nematodes"Soil & Fertilizer, NOBUNJYO, March Issue. (2000)

N. Ishibashi：“养分矿化：土壤无脊椎动物的作用；原生动物、Enchytraeidae 和线虫”土壤

Ali,R.,Y.Fukutoku,& N.Ishibashi: "Effect of Ditylenchus angustus on the growth of the rice plant" Jpn.J.Nematol.27. 51-65 (1997)

阿里，R.，Y.福德，

Ishibashi,N.,Saramoto,M.,& Ali,M.R.: "mass-production of fungivorous nematode,Aphelenchus avenae Bastian 1865,on industrial vegetable/animal wastes."Japanese Journal of Nematology. 30(in press). (2000)

石桥，N.，萨拉本，M.，