Development of the Simulated Moving-Bed Reactor Equipped with Membrane-Type Enzyme Reactors

膜式酶反应器模拟移动床反应器的研制

• 批准号: 09450291
• 负责人: HASHIMOTO Kenji
• 金额: $ 9.02万
• 依托单位: KYOTO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09450291/
• 关键词: Simulated Moving Bed; Reaction and Separation; Chromatographic Reactor; Lactosucrose; beta-Fructofuranosidase; Immobilized Enzyme; Membrane-type enzyme reactor; Numerical Simulation; 模型酵素反応器; 酵素反応; クロマトグラフィー

The objective of this study is to develop a new process by combining the enzyme reactors and simulated moving-bed adsorber and to achieve the high-yield production of lactosucrose (4^G-beta-D-galactosyl sucrose).By carrying out the batch reaction experiments using the enzyme solution, it was found that the transfructosylation followed the ordered bi bi mechanism and the hydrolyses of sucrose and lactosucrose took place simultaneously. The reaction rates were formulated from the obtained reaction mechanism. The reaction experiments using the enzyme immobilized on resin particles as well as the enzyme trapped in the hollow-fiber ultrafiltration membrane were also carried out. By immobilizing the enzyme on the resin, the lactosucrose selectivity was reduced, In the follow-fiber membrane reactor, the mixing conditions reached complete mixing, which slightly reduced the lactosucrose selectivity.The adsorption isotherms and volumetric mass transfer coefficients of each saccharide were determined in single-column breakthrough experiments.Simultaneous reaction and separation experiments were carried out by using the simulated moving-bed reactor with enzyme solution as well as with immobilized enzyme. The selectivity and yield of lactosucrose were 71% and 51% respectively when the enzyme solution was used at 50。C.The lactosucrose selectivity was 60% when the immobilized enzyme was used at 50。C.The results of the numerical simulation of the simulated moving-bed reactor demonstrated that the reverse reaction of the transfructosylation is successfully suppressed by applying the simulated moving-bed technology to this reaction system. It was also found that the sucrose conversion in each reaction column has to be reduced and the number of the reaction columns has to be increased in order to obtain a high yield of lactosucrose in the simulated moving-bed reactor equipped with the immobilized enzyme columns.

The objective of this study is to develop a new process by combining the enzyme reactors and simulated moving-bed adsorber and to achieve the high-yield production of lactosucrose (4^G-beta-D-galactosyl sucrose).By carrying out the batch reaction experiments using the enzyme solution, it was found that the transfructosylation followed the ordered bi bi mechanism and the hydrolyses of sucrose and乳糖发生了简单。反应速率是从获得的反应机理中提出的。还进行了使用固定在树脂颗粒上的酶以及被困在空心纤维超滤膜中的酶。通过将酶固定在树脂上，在后续纤维膜反应器中降低了乳糖核的选择性，混合条件达到了完全混合，从而稍微降低了乳糖核的选择性。吸附等异位液和体积的质量质量转移系数是单一的释放实验。通过使用酶溶液以及固定酶的模拟移动反应器。当使用50时酶溶液使用酶溶液时，乳糖乳糖的选择性和产量分别为71％和51％。C。当使用固定的酶时，乳酸酶的选择性为60％。在50处使用C. C. C. c。通过模拟反应器的数值模拟的数值模拟，该模拟反应器通过抑制了这种反射的反应，该反应抑制了该系统的反射系统，该系统成功地抑制了移动的系统。还发现，必须减少每个反应柱中的蔗糖转化率，并且必须增加反应柱的数量，以便在模拟的移动床反应器中获得高产量，配备有固定的酶柱。