Environmental factors controlling the production of recombinant proteins in Nicotiana benthamiana plants grown hydroponically in mobile gullies

控制流动沟渠水培植物重组蛋白产生的环境因素

• 批准号: 511844-2017
• 负责人: Pepin, Steeve
• 金额: $ 28.3万
• 依托单位: Université Laval
• 依托单位国家: 加拿大
• 项目类别: Collaborative Research and Development Grants
• 财政年份: 2018
• 资助国家: 加拿大
• 起止时间: 2018-01-01 至 2019-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=661929
• 关键词: Environmental; factors; controlling; production; recombinant

The overall goal of this project is to determine the main factors regulating transient expression and accumulation of recombinant vaccine antigens and other biopharmaceuticals in Nicotiana benthamiana (NB) cultivated hydroponically in moving gullies. This type of cultivation system offers great potential for large-scale production of NB plants, a requirement for our partner to the project, Medicago inc., whose objective is to produce each year tens of million doses of quadrivalent seasonal flu vaccines. Medicago has improved the NB expression platform considerably over the last decade. A vaccine antigen, the influenza virus hemagglutinin H1, expressed in leaves of agroinfiltrated NB plants is accumulated in membrane-enveloped virus-like particles, which are later purified by an extraction-clarification process. Our goal will be to establish, for NB plants grown and incubated in a gully system, the best crop management program (i.e. irrigation regime as a function of plant growth, attributes of the nutrient solution, recirculation and disinfection) for maximal production of recombinant proteins. The project will contribute to basic knowledge on this model plant in a context of increased manufacturing capacity, and provide Medicago and other organizations with useful data and guidelines on cultural practices to achieve high recombinant protein yield and quality in NB plants grown hydroponically in gullies. It will also contribute to the training of highly qualified graduate students and personnel in molecular biotechnology, a R&D sector of economic importance in Canada.

该项目的总体目标是确定在移动沟渠水培烟草（NB）中调节重组疫苗抗原和其他生物药物瞬时表达和积累的主要因素。这种类型的培养系统为大规模生产 NB 植物提供了巨大的潜力，这是我们该项目的合作伙伴 Medicago Inc. 的要求，该项目的目标是每年生产数千万剂四价季节性流感疫苗。在过去十年中，Medicago 显着改进了 NB 表达平台。疫苗抗原流感病毒血凝素 H1 在农杆菌渗透的 NB 植物的叶子中表达，积累在膜包膜的病毒样颗粒中，随后通过提取-澄清过程进行纯化。我们的目标是为在沟渠系统中生长和孵化的 NB 植物建立最佳作物管理计划（即根据植物生长、营养液属性、再循环和消毒的灌溉制度），以最大程度地生产重组蛋白。该项目将在提高生产能力的背景下，为该模型植物提供基础知识，并为 Medicago 和其他组织提供有关栽培实践的有用数据和指南，以在沟壑中水培的 NB 植物中实现高重组蛋白产量和质量。它还将有助于培养分子生物技术领域的高素质研究生和人员，分子生物技术是加拿大具有重要经济意义的研发部门。