Bactericidal components and mechanisms of an advanced oxidation system for water purification

水净化高级氧化系统的杀菌成分和机制

• 批准号: 518278-2017
• 负责人: Gaenzle, Michael
• 金额: $ 5.83万
• 依托单位: University of Alberta
• 依托单位国家: 加拿大
• 项目类别: Collaborative Research and Development Grants
• 财政年份: 2018
• 资助国家: 加拿大
• 起止时间: 2018-01-01 至 2019-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=651322
• 关键词: Bactericidal; components; mechanisms; advanced; oxidation

Biolargo Water Inc. of Edmonton has provided proof of concept that its patented Advanced Oxidation System (AOS) eliminates bacteria in water. The system thus has the potential to provide an economically advantageous alternative to water disinfection, and for removal of organic compounds in water. However, the mechanisms of the bactericidal effect of the system remain unknown. Different from current methods for water disinfection and purification, which are mainly based on oxidative stress imposed by UV light, chlorine, or iodine, Biolargo's AOS ability to rapidly kill microorganisms is based on a combination of oxidative stress that is induced by reactive iodine species, acid and alkaline stress, and stress imposed by iodine. While bacterial resistance to these individual stressors is well understood, the effect of the combined application of several of these lethal or sublethal stressors on microbial physiology and survival is unknown. Optimization of the chemical composition of the treatment solution as well as improvement of the process, equipment design, and scale up of the process requires increased knowledge on the bactericidal mechanisms. This proposal will assess bactericidal mechanisms of the AOS by evaluating the sensitivity of selected mutants of Escherichia coli. Explorative experimentation during an ENGAGE project provided proof of concept that this experimental approach generates relevant knowledge, and additionally indicated that the composition of the treatment fluid with respect to organic compounds, ions, and chlorine is an important parameter with respect to the bactericidal effect of the AOS. This project will provide a comprehensive assessment of the oxidative ion species generated by the AOS and the bactericidal mechanisms. The knowledge generated in this project will be used by the supporting organization for improved equipment and process design, and application development.

埃德蒙顿的 Biolargo Water Inc. 提供了概念证明，证明其获得专利的高级氧化系统 (AOS) 可以消除水中的细菌。因此，该系统有可能提供一种经济上有利的水消毒替代方案，并用于去除水中的有机化合物。然而，该系统的杀菌作用机制仍不清楚。与目前的水消毒和净化方法主要基于紫外线、氯或碘施加的氧化应激不同，Biolargo 的 AOS 快速杀死微生物的能力是基于活性碘诱导的氧化应激的组合，酸和碱胁迫以及碘造成的胁迫。虽然细菌对这些单独的应激源的抵抗力已被充分了解，但联合应用这些致死或亚致死应激源中的几种对微生物生理学和生存的影响尚不清楚。处理溶液的化学成分的优化以及工艺的改进、设备设计和工艺的放大需要增加对杀菌机制的了解。该提案将通过评估选定的大肠杆菌突变体的敏感性来评估 AOS 的杀菌机制。 ENGAGE 项目期间的探索性实验提供了概念证明，即该实验方法产生了相关知识，并且还表明处理液中有机化合物、离子和氯的成分是关于杀菌效果的重要参数。 AOS。该项目将对 AOS 产生的氧化离子种类和杀菌机制进行全面评估。支持组织将利用该项目中产生的知识来改进设备和工艺设计以及应用程序开发。