Neurophotometry system: a cost-efficient, versatile tool for behavioural circuits analyses

神经光度测量系统：用于行为回路分析的经济高效的多功能工具

• 批准号: RTI-2019-00778
• 负责人: Bambico, Francis
• 金额: $ 3.85万
• 依托单位: Memorial University of Newfoundland
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments
• 财政年份: 2018
• 资助国家: 加拿大
• 起止时间: 2018-01-01 至 2019-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=644561
• 关键词: Neurophotometry; system; cost; efficient; versatile

This applications requests for funds to be able to install an in vivo neurophotometry workstation system at the Psychology Department of Memorial University. This integrated system will consist of a fiber photometry core equipment and an additional optogenetics add-on. The system is a cutting-edge development in neurophysiology and behavioural circuits analyses. It allows for the real-time, high-resolution, in vivo monitoring of electrical activity on different neuronal compartments – cell body, dendrites and axons – and along discrete pathways and circuits. The optogenetics component allows for the controlled stimulation or inhibition of genetically distinct cell types, whereas fiber photometry makes it possible to detect calcium signals from emission wavelengths of calcium indicators as quantified measures of neural cell activity. This system has a great advantage over standard electrophysiological techniques because it allows for the identification and manipulation of genetically distinct neuronal subtypes and associated neural pathways and circuits. It is also cost-effective, portable and simple to operate. The requested system will be used as the centerpiece research module in my laboratory (applicant: Dr. Francis R. Bambico) to support my on-going NSERC DG-funded research program in stress neurobiology, for which the main goal is to identify the molecular substrates along the prefrontocortical - serotonergic raphe circuit that mediate stress controllability and predictability mechanisms, in a rodent model. The system will also enrich the NSERC-funded research programs of the Neuroscience faculty of the department (six other member users: Drs. Darlene Skinner, Dr. Jaqueline Blundell, Dr. Christina Thorpe, Dr. Ashlyn Swift-Gallant, Dr. Susan Walling and Dr. Ian Webb), as well as projects of other Neuroscience investigators in other departments (Dr. Mark Berry, biochemistry; Dr. Qi Yuan and Dr. Matthew Parsons, Faculty of Medicine). In addition to strengthening our research programs and for the training of HQP (graduate students and undergraduate honours projects), the requested equipment will be in routine use for teaching and instruction for our undergraduate and graduate curricular courses in neuroscience techniques, research experience, directed studies and SURA/USRA-associated activities.**

该申请要求资金能够在纪念大学心理学系安装体内神经光度测量工作站系统。该集成系统将由光纤光度测量核心设备和额外的光遗传学附加设备组成。该系统是一个尖端系统。它允许实时、高分辨率地体内监测不同神经区室（细胞体、树突和轴突）以及离散通路和电路的电活动。光遗传学组件允许对遗传上不同的细胞类型进行受控刺激或抑制，而光纤光度测定法可以检测来自钙指示剂发射波长的钙信号，作为神经细胞活动的量化测量，该系统比标准电生理技术具有很大的优势，因为。它可以识别和操纵遗传上不同的神经亚型以及相关的神经通路和回路。它还具有成本效益、便携且易于操作。所需的系统将用作我的实验室的核心研究模块（申请人： Francis R. Bambico 博士）支持我正在进行的 NSERC DG 资助的压力神经生物学研究项目，该项目的主要目标是识别沿着前额皮质 - 血清素中缝回路介导压力可控性和可预测性机制的分子底物，该系统还将丰富该系神经科学系的 NSERC 资助的研究项目（其他六名成员用户：Darlene Skinner 博士、Dr. Jaqueline Blundell、Christina Thorpe 博士、Ashlyn Swift-Gallant 博士、Susan Walling 博士和 Ian Webb 博士），以及其他部门其他神经科学研究人员的项目（Mark Berry 博士，生物化学；Qi Yuan 博士和Matthew Parsons 博士，医学院）除了加强我们的研究项目和 HQP（研究生和本科生荣誉项目）的培训外，所需的设备还将用于教学和日常使用。为我们的本科生和研究生课程提供有关神经科学技术、研究经验、定向研究和 SURA/USRA 相关活动的指导。**