Studying Dynamics and Energetics of Membrane Protein Oligomerization using Single Molecule Fluorescence

使用单分子荧光研究膜蛋白寡聚的动力学和能量学

• 批准号: RGPIN-2017-06871
• 负责人: Blunck, Rikard
• 金额: $ 3.28万
• 依托单位: Université de Montréal
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2017
• 资助国家: 加拿大
• 起止时间: 2017-01-01 至 2018-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=632370
• 关键词: Studying; Dynamics; Energetics; Membrane; Protein

The cell wall, or cell membrane, is composed of a double-layered lipid film and proteins, functional macromolecules that control electrical signals and substrate transport in and out of the cell. Membrane proteins also sense external stimuli and trigger signaling cascades in the inside. Until recently, the lipid membrane itself was considered mainly a passive matrix that separates the cell interior from the exterior. However, recently, it became increasingly clear that the composition of the lipid membrane influences the functioning of the proteins. This also relates to our nutrition; compounds such as cholesterol and omega-3 are known to enter the lipid film, alter its properties and modulate protein function. Conversely, also the proteins embedded in the lipid membrane alter its phase behaviour and form.

细胞壁或细胞膜由双层脂质膜和蛋白质、控制电信号和底物进出细胞的功能性大分子组成。膜蛋白还感知外部刺激并触发内部信号级联。直到最近，脂质膜本身主要被认为是一种将细胞内部与外部分开的被动基质。然而，最近人们越来越清楚，脂质膜的组成会影响蛋白质的功能。这也与我们的营养有关；众所周知，胆固醇和 omega-3 等化合物会进入脂质膜，改变其特性并调节蛋白质功能。相反，嵌入脂质膜中的蛋白质也会改变其相行为和形式。