Mikroevolution in pathogenen Bakterien

病原菌的微进化

• 批准号: 5110344
• 负责人: Professor Dr. Mark Achtman
• 金额: --
• 依托单位: Environmental Research Institute
• 依托单位国家: 德国
• 项目类别: Priority Programmes
• 财政年份: 1998
• 资助国家: 德国
• 起止时间: 1997-12-31 至 2003-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/5110344?language=en
• 关键词: Mikroevolution; pathogenen; Bakterien

In recent years, phylogenetic analyses of sequence data have yielded numerous exciting and novel insights on population genetic and evolutionary aspects of bacterial pathogens. This project will use phylogenetic methods to examine microevolution in Neisseria, Yersinia pestis, Yersinia enterocolitica and Escherichia coli. The taxonomic relationships of a new Neisseria species, currently N. spp., will be defined relative to N. meningitidis, N. gonorrhoeae and related commensal neisseriae by sequence analysis plus DNA-DNA hybridization. DNA regions containing unusually low levels of polymorphic sites have been found in N. meningitidis and Y. enterocolitica. These regions will be investigated by population genetic techniques such as coalescence analysis to test whether selective sweeps have recently occurred, and to determine the basic properties of such phenomena. A PCR-based, fine typing method will be developed for Y. pestis based on the variable insertion sites of IS100 that will enable recognition and subdivision of the biovars antiqua, medievalis and orientalis. A multilocus sequence typing (MLST) classification will be developed for E. coli in order to enable laboratories in different parts of the world to interact by using a common classification system.

近年来，序列数据的系统发育分析对细菌病原体的种群遗传和进化方面产生了许多令人兴奋和新颖的见解。该项目将使用系统发育方法检查奈瑟氏菌，耶尔森氏菌，小肠结肠炎和大肠杆菌的微观进化。通过序列分析加上DNA-DNA杂交，将定义一种新的奈瑟氏菌种的分类关系，即目前N. spp。的分类关系。在脑膜炎和Y. ensolecolitica中发现了含有异常含量的多态性位点的DNA区域。这些区域将通过人群遗传技术（例如结合分析）进行研究，以测试最近是否发生了选择性扫描，并确定这种现象的基本特性。将基于Y. Pestis开发一种基于PCR的精细键入方法，该方法基于IS100的可变插入位点，该位点将实现Biovars Antiqua，Medievalis和Orientalis的识别和细分。将为大肠杆菌开发多焦点序列分类（MLST）分类，以使世界各地的实验室能够使用共同的分类系统进行交互。