Generating molecular diversity by the wide range of polyketide synthases

通过多种聚酮合酶产生分子多样性

• 批准号: 22241054
• 负责人: NOGUCHI Hiroshi
• 金额: $ 26.62万
• 依托单位: University of Shizuoka
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-22241054/
• 关键词: ゲノム; 酵素工学; 生理活性; biosynthesis; 生合成; メロテルペノイド; 分子多様性; テルペン環化酵素; 酵素; テレトニン; プレニル基転移酵素; バイオテクノロジー

Novel type III PKS was isolated from Garcinia mangostana L. , which contains mainly prenylated xanthones. A key prenyltransferase involved in the biosynthesis of abundant fungal meroterpenoids derived from 3,5-dimethylorsellinic was identified. The prenylated product serves as a key intermediate in the biosynthesis of series of meroterpenoids in fungi. With Aspergillus nidlans, Metabolic engineering of yeast for heterologous production of tryprostatins and other compounds was developed through our engineered strain of Saccharomyces cerevisiae, SCKW5. Further trial to architect comprehensive system to form secondary metabolites with Chaetomium globosum is under progress.

新型III型PKS是从藤黄L.分离出来的，该L.主要含有原型Xanthone。鉴定出参与涉及3,5-二甲基硅脂的丰富真菌杂色的生物合成的关键前转移酶。婚前产物是真菌系列的生物合成中的关键中间体。借助曲霉菌尼德兰（Aspergillus nidlans），通过我们的工程酿酒酵母菌株SCKW5开发了酵母的代谢工程，用于异源生产TryProstatins和其他化合物。对建筑师综合系统的进一步试验正在进行中，形成二级代谢物，并正在进行综合球形。

项目成果

Establishing a new methodology for genome mining and biosynthesis of natural products through fungal molecular genomics

• DOI: 10.1055/s-0032-1320342
• 发表时间: 2012-07
• 期刊: Planta Medica
• 影响因子: 2.7
• 作者: H. Noguchi

A novel triterpene synthase gene from a monocot plant, Imperata cylindrical

来自单子叶植物白茅的新型三萜合酶基因

• 发表时间: 2012
• 作者: 池内佑一郎;喜多隆;他;荒井 雅吉;Tetsuo Kushiro
• 通讯作者: Tetsuo Kushiro

Yeast, a more manageable non-ribosomal peptide mill for Spirotryprostatin B assembly

酵母，一种更易于管理的非核糖体肽研磨机，用于螺旋胰前列腺素 B 组装

• 发表时间: 2011
• 作者: 今野元貴;高梨祐希;柳川博文;吉川浩司;松本光雄;福島学;K. Watanabe
• 通讯作者: K. Watanabe

Biologically active molecules from fungi

来自真菌的生物活性分子

• 发表时间: 2012
• 作者: Hata;K.;Kato;H. & Kachi;N.;荒井 雅吉;Kenji Watanabe
• 通讯作者: Kenji Watanabe

A comprehensive overview on genomically directed assembly of aromatic polyketides and macrolide lactones using fungal megasynthases

• DOI: 10.1038/ja.2010.130
• 发表时间: 2011-05
• 期刊: The Journal of Antibiotics
• 作者: T. Saruwatari;Alex P Praseuth;Michio Sato;Kohei Torikai;H. Noguchi;Kenji Watanabe