Speciation of Taeniid cestodes (Echinococcus spp. and Taenia spp.) in the world and co-evolution of the host-parasite relationship

世界绦虫绦虫（棘球绦虫属和带绦虫属）的物种形成及宿主-寄生虫关系的共同进化

• 批准号: 21256003
• 负责人: ITO Akira
• 金额: $ 24.71万
• 依托单位: Asahikawa Medical College
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-21256003/
• 关键词: 感染症; 遺伝子; エキノコックス条虫; テニア条虫; 遺伝子分類; ミトコンドリア遺伝子; 核遺伝子; 地理拡散; 進化

Parasitic cestodes, Cestoda, Plathelminthes, have well been known to be hermatophroditic. Self-fertilization is the basic strategy for reproduction biology in cestodes. However, through our most recent studies on mitochondrial (haploid) and nuclear (diploid) DNA of two independent species infecting humans, Taenia saginata and Taenia asiatica have revealed that there are hybrids or hybrid derived adult tapeworms in Thailand and China where these species are sympatrically distributed. These data strongly reveal cross fertilization in the definitive host, humans, and launch “What is T. asiatica? Is it really independent species?" It is still under debates. Similar evidence showing cross fertilization between the intraspecies two, Asian and Afro/American genotypes of Taenia solium has been confirmed from larval stage of this parasite sampled in Madagascar. In Madagascar, we could have confirmed that the two genotypes are sympatrically distributed and also some hybrid individuals have been confirmed. Our work is the first one comparing both mitochondrial and nuclear genes in cestodes or platyhelminthes. These all data strongly suggest the cross fertilization in cestode reproduction biology. Molecular studies on Echinococcus spp. have also revealed that E. granulosus sensu lato is not a single species but 5 species, and a total of 9 independent species are included in the genus Echinococcus. Among these species, E. multilocularis is highly pathogenic and 3 (North American, Asian and European) genotypes have been known, but we have confirmed additional one, Mongolian genotype. The new information on the cestode speciation and identification has successfully been confirmed by the establishment of immuno- and molecular-diagnostic tools developed by our research team.

寄生虫，cestoda，plathelminthes众所周知是雌雄同体。自我受精是Cestodes中生物学生物学的基本策略。然而，通过我们最近关于两个独立物种感染了人类的线粒体（单倍体）和核（二倍体）DNA的研究，泰尼亚·萨吉纳塔（Taenia Saginata）和泰尼亚亚洲人（Taenia saginata）和泰尼亚亚洲人（Taenia asiatica 物种？”它仍在辩论中。类似的证据表明，从马达加斯加采样的这种寄生虫的幼体阶段证实了两种质体的内在，亚洲和美国/美国基因型之间的交叉施肥。在马达加斯加，我们可以证实这两种基因型是对称分布的，并且已经确认了一些混合动力。我们的工作是第一个比较Cestodes或Platyhelminthes中线粒体和核基因的工作。这些数据强烈表明Cestodo繁殖生物学中的交叉施肥。关于棘球菌属的分子研究。还透露，大肠杆菌sensu lato不是一个物种，而是5种，其中9种独立物种是这些物种，多孔眼大肠杆菌具有高度致病性，3种（北美，亚洲和欧洲）的基因型已知，但我们已经确认了另外一种，蒙古人的基因型。通过我们的研究团队开发的免疫和分子诊断工具，已成功确认了有关CESTODE规范和识别的新信息。

项目成果

Specific IgG Responses to Recombinant Antigen B and Em18 in Cystic and Alveolar Echinococcosis in China

• DOI: 10.1128/cvi.00466-09
• 发表时间: 2010-03-01
• 期刊: CLINICAL AND VACCINE IMMUNOLOGY
• 作者: Li, Tiaoying;Ito, Akira;Craig, Philip S.
• 通讯作者: Craig, Philip S.

Epidemiological and phylogenetic considerations regarding Echinococcus species

关于棘球绦虫属的流行病学和系统发育考虑因素

• 发表时间: 2011
• 作者: 中尾稔

Cross-fertilization and hybridization in Taenia species

带绦虫物种的异体受精和杂交

• 发表时间: 2009
• 作者: 志水理哉;伊藤弘毅;田邉浩之;波木理恵子;鷲崎弘宜;深澤良彰;Okamoto M
• 通讯作者: Okamoto M

Invited speaker on "The present situation of alveolar Echinococcosis and cystic Echinococcosis in Mongolia" at "Molecular Epidemiology"

《分子流行病学》特邀演讲者“蒙古肺泡型包虫病和囊型包虫病的现状”

• 发表时间: 2011

In: Handbook of Nucleic Acid Purification (ed. by Liu D), CRC Press, Taylor & Francis Group, Boca Raton

见：《核酸纯化手册》（刘 D 编），CRC Press，Taylor

• 发表时间: 2009
• 作者: Okamoto M;Ito A.
• 通讯作者: Ito A.