Proton pump ATPase as nano motors

质子泵 ATP 酶作为纳米电机

• 批准号: 13142204
• 负责人: FUTAI Masamistu
• 金额: $ 51.01万
• 依托单位: Microbial Chemistry Research Center (2004-2005)Osaka University (2001-2003)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research on Priority Areas
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-13142204/
• 关键词: ATP; F-TPase; V-ATPase; Lisosom; nano machine; nano motors; プロトンポンプ; V型ATPase; ATP合成酵素; インスリン; 蛋白質; 生体分子; 酵素; 細胞組織; F型ATPase; 分泌顆粒; ランゲルハンス島

Three classes of proton pumping ATPase are known : P-TPase, F-TPase and V-ATPase. F-ATPase (ATP synthase) and V-ATP(prothn pump of endomembrance organelles such as lysosome and endosome) are closely similar in subunit structure and mechanism. They couples proton transport and ATPase chemistry through subunit rotation. Our extensive research results are showing:(1) Purified and membrane-bound F-ATPases are rotating during ATP hydrolysis. Their rotations are stochastic, and driven by hinge domain of the β subunit. (2) V-ATPases are also rotating proton pump, and their diverse isoforms specific for unique cells were found. (3) V-ATPase has domains required for specific localization and possibly for sensing organeller luminal pH. These results have been widely recognized in the field biochemistry and cell biology.

已知三类质子泵送ATPase：P-TPase，F-TPase和V-ATPase。在亚基结构和机制中，F-ATPase（ATP合酶）和V-ATP（溶酶体和内体等内膜细胞器的普罗斯泵）非常相似。他们通过亚基旋转对质子转运和ATPase化学伴侣。我们广泛的研究结果表明：（1）在ATP水解过程中，纯化和结合膜的F-ATP酶正在旋转。它们的旋转是随机的，并由β亚基的铰链域驱动。 （2）V-ATP酶也是旋转的质子泵，并发现了针对独特细胞的潜水员同工型。 （3）V-ATPases具有特定定位所需的域，并且可能是传感带管腔腔pH的域。这些结果在田间生物化学和细胞生物学中得到了广泛认可。

项目成果

M.Tanabe, K.Nishio, Y.Iko, Y.Sambongi, A.Iwamoto-Kihara, H.Omote, I.Ueda, Y.Wada, M.Futai: "Rotation of a complex of the γ subunit and c ring of Escherichia coli. ATP synthase : the rotor and stator are interchangeable"J.Biol.Chem.. 276. 15269-15274 (2001

M.Tanabe、K.Nishio、Y.Iko、Y.Sambongi、A.Iwamoto-Kihara、H.Omote、I.Ueda、Y.Wada、M.Futai：“γ 亚基和 c 环复合物的旋转大肠杆菌的 ATP 合酶：转子和定子可互换”J.Biol.Chem.. 276. 15269-15274 (2001)

G.-H.Sun Wada, Y.Murata, M.Namba, A.Yamamoto, Y.Wada, Y., Futai, M.: "Mouse proton pump ATPase C subunit Isoforms (C2-a and C2-b) specifically expressed in kidney and lung"J Biol Chem.. 278. 44843-44851 (2003)

G.-H.Sun Wada、Y.Murata、M.Namba、A.Yamamoto、Y.Wada, Y.、Futai, M.：“小鼠质子泵 ATP 酶 C 亚基异构体（C2-a 和 C2-b）

A proton pump ATPase with testis-specific El subunit isoform required for acrosome acidification,

具有顶体酸化所需的睾丸特异性 El 亚基亚型的质子泵 ATP 酶，

• 发表时间: 2001
• 期刊: J. Biol. Chem. 277
• 作者: Masamistu Futai.;Ge-Hong Sun-Wada
• 通讯作者: Ge-Hong Sun-Wada

Rotation of a complex of the γ subunit and c ring of Escherichia coli ATP synthase : the rotor and stator are interchangeable.

大肠杆菌 ATP 合酶的 γ 亚基和 c 环复合体的旋转：转子和定子可互换。

• 发表时间: 2001
• 期刊: J.Biol.Chem. 276
• 作者: M;Futai.
• 通讯作者: Futai.

Sodium and sulfate ion transport in yeast vacuole,

酵母液泡中钠和硫酸根离子的运输，

• 发表时间: 2002
• 期刊: J. Biochem. 131
• 作者: Masamistu Futai.;Yo Wada
• 通讯作者: Yo Wada